https://scholars.lib.ntu.edu.tw/handle/123456789/551264
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Tsai, Tsuimin | en_US |
dc.contributor.author | RUEY-LONG HONG | en_US |
dc.contributor.author | JUI-CHANG TSAI | en_US |
dc.contributor.author | PEI-JEN LOU | en_US |
dc.contributor.author | Ling, I-Fang | en_US |
dc.contributor.author | Chen, Chin-Tin | en_US |
dc.creator | Tsai T.;Ruey-Long Hong;Tsai J.-C.;Lou P.-J.;Ling I.-F.;Chen C.-T. | - |
dc.date.accessioned | 2021-03-09T06:46:12Z | - |
dc.date.available | 2021-03-09T06:46:12Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0196-8092 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347762872&doi=10.1002%2flsm.10246&partnerID=40&md5=e96ca3642a64983c6958f37bc569f1db | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/551264 | - |
dc.description.abstract | Background and Objectives: Photodynamic therapy (PDT) has been proposed as an alternative approach in overcoming multidrug resistance (MDR) phenotype. To verify whether 5-aminolevulinic acid (ALA)-mediated PDT is effective in MDR cells, we studied the protoporphyrin IX (PpIX) content, intracellular localization, and phototoxicity in human breast cancer cells MCF-7 and derived MDR subline, MCF-7/ADR. Study Design/Materials and Methods: The fluorescence kinetics of ALA-induced PpIX was evaluated by spectrofluorometer. The phototoxicity of MCF-7 and MCF-7/ADR cells was determined by tetrazolium (MTT) assays and clonogenic assay. Furthermore, Annexin V and propidium iodide (PI) binding assays were performed to analyze the characteristics of cell death after ALA-PDT. Results: MCF-7/ADR accumulated a lower level of PpIX as compared to parental MCF-7 cells. Significant phototoxicity was observed in MCF-7 and increased in a fluence-dependent manner with LD50 around 8 J/cm 2. Compared to its parental counterpart, MCF-7/ADR cells were less sensitive to ALA photodynamic treatment and PDT-induced cytotoxicity did not increase in a dose responsive manner as the concentration of ALA increased or the fluence of light increased. ALA-PDT was less effective for MCF-7/ADR cells than MCF-7 cells even under the condition when these two cell lines contained the similar amounts of PpIX. Conclusions: These results indicate that, except for the MDR related characteristics, MCF-7/ADR cells might possess intrinsic mechanisms that render them less sensitive to ALA-PDT induced phototoxicity. | - |
dc.relation.ispartof | Lasers in Surgery and Medicine | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; aminolevulinic acid; lipocortin 5; propidium iodide; protoporphyrin; article; cell line; cell strain MCF 7; cellular distribution; clonogenic assay; controlled study; cytotoxicity; dose response; fluorescence; human; human cell; light; multidrug resistance; photodynamic therapy; photosensitivity; phototoxicity; priority journal; spectrofluorometry; Aminolevulinic Acid; Cell Death; Cell Line, Tumor; Humans; Mitochondria; Photochemotherapy; Photosensitizing Agents | - |
dc.title | Effect of 5-Aminolevulinic Acid-Mediated Photodynamic Therapy on MCF-7 and MCF-7/ADR Cells | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/lsm.10246 | - |
dc.identifier.pmid | 14755426 | - |
dc.identifier.scopus | 2-s2.0-0347762872 | - |
dc.relation.pages | 62-72 | - |
dc.relation.journalvolume | 34 | - |
dc.relation.journalissue | 1 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Otolaryngology | - |
crisitem.author.dept | Otolaryngology-NTUH | - |
crisitem.author.orcid | 0000-0001-8100-7914 | - |
crisitem.author.orcid | 0000-0003-2723-6841 | - |
crisitem.author.orcid | 0000-0002-3383-8593 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 腫瘤醫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。