https://scholars.lib.ntu.edu.tw/handle/123456789/551899
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hsieh, Ting-Yu | en_US |
dc.contributor.author | Vyas, Sunil | en_US |
dc.contributor.author | JUI-CHING WU | en_US |
dc.contributor.author | YUAN LUO | en_US |
dc.date.accessioned | 2021-03-11T07:46:23Z | - |
dc.date.available | 2021-03-11T07:46:23Z | - |
dc.date.issued | 2020-12-01 | - |
dc.identifier.issn | 16717694 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/551899 | - |
dc.description.abstract | Three-dimensional (3D) imaging of living organisms requires fine optical sectioning and high-speed image acquisition, which can be achieved by light sheet fluorescence microscopy (LSFM). However, orthogonal illumination and detection arms in the LSFM system make it bulky. Here, we propose and demonstrate the application of a volume holographic optical element (photopolymer-based volume holographic grating) for designing a compact LSFM system, called a volume holographic LSFM (VHLSFM). Using the VHLSFM, we performed in vivo imaging of Caenorhabditis elegans (C. elegans) and observed high-contrast optically sectioned fluorescence images of the oocytes and embryonic development in real time for 3D imaging. | en_US |
dc.language.iso | animation | en_US |
dc.relation.ispartof | Optics letters | en_US |
dc.title | Volume holographic optical element for light sheet fluorescence microscopy | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1364/OL.413204 | - |
dc.identifier.pmid | 33258841 | - |
dc.identifier.scopus | 2-s2.0-85097013371 | - |
dc.identifier.url | https://scholars.lib.ntu.edu.tw/handle/123456789/549887 | - |
dc.relation.journalvolume | 45 | en_US |
dc.relation.journalissue | 23 | en_US |
dc.relation.pageend | 6481 | en_US |
item.languageiso639-1 | animation | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Clinical Laboratory Sciences and Medical Biotechnology | - |
crisitem.author.dept | Medical Device and Imaging | - |
crisitem.author.dept | Program for Precision Health and Intelligent Medicine | - |
crisitem.author.orcid | 0000-0002-3880-9652 | - |
crisitem.author.orcid | 0000-0001-9776-7897 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | Graduate School of Advanced Technology | - |
Appears in Collections: | 醫學檢驗暨生物技術學系 |
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