https://scholars.lib.ntu.edu.tw/handle/123456789/551900
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Bai, Xiaofei | en_US |
dc.contributor.author | Huang, Leng-Jie | en_US |
dc.contributor.author | Chen, Sheng-Wen | en_US |
dc.contributor.author | Nebenfuehr, Benjamin | en_US |
dc.contributor.author | Wysolmerski, Brian | en_US |
dc.contributor.author | JUI-CHING WU | en_US |
dc.contributor.author | Olson, Sara K | en_US |
dc.contributor.author | Golden, Andy | en_US |
dc.contributor.author | Wang, Chao-Wen | en_US |
dc.creator | Bai, Xiaofei;Huang, Leng-Jie;Chen, Sheng-Wen;Nebenfuehr, Benjamin;Wysolmerski, Brian;Jui-Ching Wu;Olson, Sara K;Golden, Andy;Wang, Chao-Wen | - |
dc.date.accessioned | 2021-03-11T07:47:37Z | - |
dc.date.available | 2021-03-11T07:47:37Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 0950-1991 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/551900 | - |
dc.description.abstract | Seipin, an evolutionary conserved protein, plays pivotal roles during lipid droplet (LD) biogenesis and is associated with various human diseases with unclear mechanisms. Here, we analyzed Caenorhabditis elegans mutants deleted of the sole SEIPIN gene, seip-1 Homozygous seip-1 mutants displayed penetrant embryonic lethality, which is caused by the disruption of the lipid-rich permeability barrier, the innermost layer of the C. elegans embryonic eggshell. In C. elegans oocytes and embryos, SEIP-1 is associated with LDs and is crucial for controlling LD size and lipid homeostasis. The seip-1 deletion mutants reduced the ratio of polyunsaturated fatty acids (PUFAs) in their embryonic fatty acid pool. Interestingly, dietary supplementation of selected n-6 PUFAs rescued the embryonic lethality and defective permeability barrier. Accordingly, we propose that SEIP-1 may maternally regulate LD biogenesis and lipid homeostasis to orchestrate the formation of the permeability barrier for eggshell synthesis during embryogenesis. A lipodystrophy allele of seip-1 resulted in embryonic lethality as well and could be rescued by PUFA supplementation. These experiments support a great potential for using C. elegans to model SEIPIN-associated human diseases. | en_US |
dc.language.iso | animation | en_US |
dc.publisher | COMPANY BIOLOGISTS LTD | en_US |
dc.relation.ispartof | Development (Cambridge, England) | en_US |
dc.subject | Eggshell; Fatty acid; Lipid droplet; PUFAs; Permeability barrier; Seipin | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | fat droplet; membrane protein; omega 6 fatty acid; seipin 1; unclassified drug; Caenorhabditis elegans protein; fat droplet; unsaturated fatty acid; adult; allele; animal cell; animal experiment; animal model; animal tissue; Article; biogenesis; Caenorhabditis elegans; confocal microscopy; controlled study; data analysis software; deletion mutant; diet supplementation; egg shell; embryo; embryo development; fatty acid synthesis; female; homozygosity; lethality; lipid analysis; lipid homeostasis; lipodystrophy; male; nonhuman; oocyte; permeability barrier; phenotype; priority journal; transmission electron microscopy; young adult; animal; Caenorhabditis elegans; dietary supplement; disease model; drug effect; egg shell; embryology; fertilization; gene; gene deletion; gene expression regulation; genetics; human; lipidomics; metabolism; mutation; nonmammalian embryo; ovulation; permeability; Saccharomyces cerevisiae; ultrastructure; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Dietary Supplements; Disease Models, Animal; Egg Shell; Embryo, Nonmammalian; Fatty Acids, Unsaturated; Fertilization; Gene Deletion; Gene Expression Regulation, Developmental; Genes, Helminth; Humans; Lipid Droplets; Lipidomics; Mutation; Oocytes; Ovulation; Permeability; Saccharomyces cerevisiae | - |
dc.title | Loss of the seipin gene perturbs eggshell formation in Caenorhabditis elegans | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1242/dev.192997 | - |
dc.identifier.pmid | 32820022 | - |
dc.identifier.scopus | 2-s2.0-85093705873 | - |
dc.identifier.isi | WOS:000589171400008 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85093705873 | - |
dc.relation.journalvolume | 147 | en_US |
dc.relation.journalissue | 20 | en_US |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | animation | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
crisitem.author.dept | Clinical Laboratory Sciences and Medical Biotechnology | - |
crisitem.author.orcid | 0000-0002-3880-9652 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學檢驗暨生物技術學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。