https://scholars.lib.ntu.edu.tw/handle/123456789/553302
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | SZE-KWAN LIN | en_US |
dc.contributor.author | YEN-PING KUO | en_US |
dc.contributor.author | Wang J.-S. | en_US |
dc.contributor.author | Lee J.-J. | en_US |
dc.contributor.author | Wang C.-C. | en_US |
dc.contributor.author | Huang S. | en_US |
dc.contributor.author | CHIA-TUNG SHUN | en_US |
dc.contributor.author | Hong C.-Y. | en_US |
dc.date.accessioned | 2021-03-18T02:03:29Z | - |
dc.date.available | 2021-03-18T02:03:29Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0099-2399 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/553302 | - |
dc.description.abstract | Increased levels of interleukin-1 (IL)-1, tumor necrosis factor-α (TNF-α), IL-6, and prostaglandin E2 (PGE2) have been detected in inflamed pulp tissue. To gain further insight into the molecular pathogenesis of pulpitis, we investigated the effects of IL-1α or TNF-α and PGE2, either alone or in combination on IL-6 and cyclooxygenase (COX)-2 messenger RNA (mRNA) production in cultured human dental pulp (HDP) fibroblasts. Exposure of HDP fibroblasts to IL-1α or TNF-α resulted in elevated levels of IL-6 (?3.4 to ?10.4-fold) and COX-2 (?5 to ?6.2-fold) mRNA. Simultaneous addition of IL-1α and PGE2 or TNF-α and PGE2 to the cultures significantly reduced the cytokine-induced IL-6 mRNA synthesis ranging from 45% to 65%. However, indomethacin enhanced the cytokine-stimulated IL-6 mRNA synthesis by ?1.7 to ?3.4-fold. This action could be reversed by exogenous PGE 2. In contrast, PGE2 or indomethacin failed to modify the stimulatory effect of IL-1α or TNF-α on COX-2 gene expression. Because excessive levels of IL-6 and prostaglandins have been connected with the pathogenesis of several inflammatory diseases, our results suggest the involvement of HDP fibroblasts in the development of pulpitis via producing IL-6 and COX-2. Furthermore, expression of IL-6 and COX-2 genes in this cell seems to be differentially regulated by cytokines through prostaglandin-dependent and -independent pathways. Copyright ? 2002 by The American Association of Endodontists. | - |
dc.relation.ispartof | Journal of Endodontics | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | cyclooxygenase 2; cyclooxygenase 2 inhibitor; cytokine; indometacin; interleukin 1; interleukin 6; isoenzyme; membrane protein; messenger RNA; nonsteroid antiinflammatory agent; prostaglandin E2; prostaglandin synthase; prostaglandin synthase inhibitor; PTGS2 protein, human; tumor necrosis factor alpha; article; biosynthesis; cell culture; cytology; drug effect; gene expression regulation; human; metabolism; Northern blotting; pulpitis; tooth pulp; Anti-Inflammatory Agents, Non-Steroidal; Blotting, Northern; Cells, Cultured; Cyclooxygenase 2; Cyclooxygenase 2 Inhibitors; Cyclooxygenase Inhibitors; Cytokines; Dental Pulp; Dinoprostone; Gene Expression Regulation; Humans; Indomethacin; Interleukin-1; Interleukin-6; Isoenzymes; Membrane Proteins; Prostaglandin-Endoperoxide Synthases; Pulpitis; RNA, Messenger; Tumor Necrosis Factor-alpha | - |
dc.title | Differential regulation of interleukin-6 and inducible cyclooxygenase gene expression by cytokines through prostaglandin-dependent and -independent mechanisms in human dental pulp fibroblasts | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1097/00004770-200203000-00013 | - |
dc.identifier.pmid | 12017180 | - |
dc.identifier.scopus | 2-s2.0-0036513026 | - |
dc.relation.pages | 197-201 | - |
dc.relation.journalvolume | 28 | - |
dc.relation.journalissue | 3 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Dentistry | - |
crisitem.author.dept | School of Dentistry | - |
crisitem.author.dept | Dentistry-NTUH | - |
crisitem.author.dept | Dentistry | - |
crisitem.author.dept | School of Dentistry | - |
crisitem.author.dept | Dentistry-NTUH | - |
crisitem.author.dept | Forensic Medicine | - |
crisitem.author.orcid | 0000-0002-7969-0280 | - |
crisitem.author.orcid | 0000-0001-8321-2575 | - |
crisitem.author.orcid | 0000-0002-0468-4468 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 法醫學科所 |
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