https://scholars.lib.ntu.edu.tw/handle/123456789/556815
標題: | Genetic determinants of capsular serotype K1 of Klebsiella pneumoniae causing primary pyogenic liver abscess | 作者: | Chuang Y.-P. CHI-TAI FANG Lai S.-Y. SHAN-CHWEN CHANG JIN-TOWN WANG |
公開日期: | 2006 | 卷: | 193 | 期: | 5 | 起(迄)頁: | 645-654 | 來源出版物: | Journal of Infectious Diseases | 摘要: | Background. Primary pyogenic liver abscess (PLA) caused by Klebsiella pneumoniae is an emerging infectious disease. Capsular serotype K1 and the magA gene have been reported to be associated with this disease. Methods. The prevalence of magA was determined by polymerase chain reaction (PCR). The sequences of the magA flanking region were completed by inverse PCR and direct sequencing. Serotyping was performed by double immunodiffusion. Insertion mutations and trans-complementation were used to define the K1 genetic determination region. Results. Thirty-five of 42 strains from patients with PLA were magA positive, whereas only 1 of 32 non-PLA strains was magA positive. All 36 magA-positive strains were serotype K1, and the 38 magA-negative strains were not (36/36 vs. 0/38; P < .0001). Sequencing of the magA flanking region revealed a putative capsular polysaccharide synthesis (cps) region; this region was 25 kb in length and contained 20 open reading frames (ORFs); of these ORFs, 9 were cotranscribed as part of an operon and differed from both MGH78578 and the Chedid strain. Mutation of 4 genes in this region turned the mutant strains anti-K1 negative. Trans-complementation restored the K1 phenotype. Conclusions. The operon containing magA is responsible for capsular serotype K1 of K. pneumoniae. Several loci in the operon are unique determinants of K1 strains. ? 2006 by the Infectious Diseases Society of America. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33144464254&doi=10.1086%2f499968&partnerID=40&md5=13421268a321553f2de0beeed0f732b1 https://scholars.lib.ntu.edu.tw/handle/123456789/556815 |
ISSN: | 0022-1899 | DOI: | 10.1086/499968 | SDG/關鍵字: | bacterial polysaccharide; article; bacterial gene; bacterial membrane; bacterial strain; carbohydrate synthesis; controlled study; DNA flanking region; gene insertion; gene locus; gene mutation; gene sequence; genetic complementation; genetic transcription; genetics; immunodiffusion; Klebsiella pneumoniae; magA gene; nonhuman; nucleotide sequence; open reading frame; operon; phenotype; polymerase chain reaction; prevalence; priority journal; pyogenic liver abscess; serotype; serotyping; Antigens, Bacterial; Bacterial Proteins; DNA, Bacterial; Genetic Complementation Test; Humans; Klebsiella Infections; Klebsiella pneumoniae; Liver Abscess, Pyogenic; Mutagenesis, Insertional; Open Reading Frames; Operon; Polymerase Chain Reaction; Polysaccharides, Bacterial; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sequence Analysis, DNA; Serotyping; Transcription, Genetic; Virulence Factors |
顯示於: | 流行病學與預防醫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。