https://scholars.lib.ntu.edu.tw/handle/123456789/556997
標題: | Proteomic Analysis of Metastasis-Specific Biomarkers in Pancreatic Cancer: Galectin-1 Plays an Important Metastatic Role in Pancreatic Cancer | 作者: | Tung C.-L. Lin M.-W. Hu R.-Y. Chien Y.-C. Liao E.-C. Lin L.-H. Chung T.-W. Wei Y.-S. Tsai Y.-T. Chen H.-Y. Chou H.-C. WEN-HUNG KUO MEI-LAN KO Chang S.-J. Lee Y.-R. Chan H.-L. |
關鍵字: | DIGE; galectin-1; Metastasis; Pancreatic Cancer; Proteomics | 公開日期: | 2020 | 出版社: | Elsevier B.V. | 卷: | 186 | 來源出版物: | Journal of Pharmaceutical and Biomedical Analysis | 摘要: | Cancer metastasis is the major cause of death in pancreatic cancer. We have established a pair of pancreatic ductal adenocarcinoma cell line, PANC1 and invasive PANC1-I5, as a model system toinvestigate the metastatic mechanism as well as potential therapeutic targets in pancreatic cancer. We used proteomic analysis based on two-dimensional differential gel electrophoresis (2D-DIGE) and matrix?assisted laser desorption ionization time?of?flight mass spectrometry (MALDI-TOF MS) to examine the global protein expression alterations between PANC1 and PANC1-I5. Proteomic study revealed that 88 proteins are differentially expressed between PANC1-I5 and PANC1 cells, and further functional evaluations through protein expression validation, gene knockout, migration and invasion analysis revealed that galectin-1 is one of the potential players in modulating pancreatic cancer metastasis. To conclude, we have identified numerous proteins might be associated with pancreatic cancer invasiveness in the pancreatic cancer model. ? 2020 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084392151&doi=10.1016%2fj.jpba.2020.113300&partnerID=40&md5=7e0a60b60e2b459c690d63323e873d51 https://scholars.lib.ntu.edu.tw/handle/123456789/556997 |
ISBN: | 32413824 | ISSN: | 0731-7085 | DOI: | 10.1016/j.jpba.2020.113300 | SDG/關鍵字: | galectin 1; proteome; tumor marker; galectin 1; LGALS1 protein, human; tumor marker; Article; cancer patient; controlled study; gene knockout; human; human cell; matrix assisted laser desorption ionization time of flight mass spectrometry; PANC-1 cell line; panc1 i5 cell line; pancreas cancer; pancreas metastasis; pancreatic ductal carcinoma cell line; priority journal; protein analysis; protein expression; proteomics; two dimensional difference gel electrophoresis; adenocarcinoma; cell motion; gene expression regulation; genetics; matrix-assisted laser desorption-ionization mass spectrometry; metabolism; metastasis; pancreas carcinoma; pancreas tumor; pathology; proteomics; tumor cell line; tumor invasion; Adenocarcinoma; Biomarkers, Tumor; Carcinoma, Pancreatic Ductal; Cell Line, Tumor; Cell Movement; Galectin 1; Gene Expression Regulation, Neoplastic; Gene Knockout Techniques; Humans; Neoplasm Invasiveness; Neoplasm Metastasis; Pancreatic Neoplasms; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Two-Dimensional Difference Gel Electrophoresis |
顯示於: | 醫學系 |
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