https://scholars.lib.ntu.edu.tw/handle/123456789/561155
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lai C.-C. | en_US |
dc.contributor.author | Tan C.-K. | en_US |
dc.contributor.author | Lin S.H. | en_US |
dc.contributor.author | Liao C.-H. | en_US |
dc.contributor.author | Chou C.-H. | en_US |
dc.contributor.author | YU-TSUNG HUANG | en_US |
dc.contributor.author | PO-REN HSUEH | en_US |
dc.date.accessioned | 2021-05-18T05:57:05Z | - |
dc.date.available | 2021-05-18T05:57:05Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0012-3692 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/561155 | - |
dc.description.abstract | Background: Isolation of Mycobacterium tuberculosis (MTB) from the clinical specimens of patients with suspected TB remains the gold standard for diagnosis of TB. However, false-positive MTB cultures can occur as a result of laboratory contamination. Methods: After reviewing the medical records of 400 TB cases identified during January 2008 to January 2009 by the infection control unit of a university-affiliated hospital in Taipei, Taiwan, five patients were considered as clinically suspected false-positive cases and were referred to a mycobacteriology laboratory for confirmation. Spoligotyping and mycobacterial interspersed repetitive unit-variable number tandem repeat analyses were performed for all the suspected isolates and all other isolates cultured on the same day as the five suspected isolates. Results: Three cases were confirmed as false-positive culture cases based on the laboratory investigation. The culture from one of these cases (index case 1) grew multidrug-resistant TB. Another patient (index case 2) received an extended course of anti-TB treatment after he was considered to have failed treatment because of the false-positive MTB culture result. No anti-TB medication was given for index case 3. All three cases with false-positive cultures had only one positive culture specimen among multiple consecutive specimens submitted for cultures. In addition, specimens of the false-positive cultures were all negative for acid-fast smears. Conclusions: False-positive MTB cultures should be suspected in the following situations: when growth is observed on only one specimen among multiple specimens submitted; when it is positive in only one culture medium, especially in broth; or when there is only one specimen submitted. False-positive MTB cultures can be further confirmed with modern molecular typing techniques. ? 2010 American College of Chest Physicians. | - |
dc.relation.ispartof | Chest | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | tuberculostatic agent; acid fast bacterium; adult; aged; article; bacterium contamination; bacterium culture; bacterium isolate; bacterium isolation; comorbidity; computer assisted tomography; controlled study; culture medium; drug treatment failure; female; human; infection control; laboratory diagnosis; major clinical study; male; medical record review; molecular epidemiology; molecular typing; morbidity; multidrug resistant tuberculosis; Mycobacterium tuberculosis; priority journal; spoligotyping; sputum culture; sputum smear; Taiwan; tandem repeat; thorax radiography; tuberculosis; university hospital | - |
dc.title | Molecular evidence of false-positive cultures for Mycobacterium tuberculosis in a Taiwanese hospital with a high incidence of TB | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1378/chest.09-1878 | - |
dc.identifier.scopus | 2-s2.0-77951819188 | - |
dc.relation.pages | 1065-1070 | - |
dc.relation.journalvolume | 137 | - |
dc.relation.journalissue | 5 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Laboratory Medicine-NTUH | - |
crisitem.author.dept | Laboratory Medicine | - |
crisitem.author.dept | Laboratory Medicine | - |
crisitem.author.dept | Laboratory Medicine-NTUH | - |
crisitem.author.orcid | 0000-0002-7805-4178 | - |
crisitem.author.orcid | 0000-0002-7502-9225 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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