Diagnosis of tuberculosis by an enzyme-linked immunospot assay for interferon-γ
Journal
Emerging Infectious Diseases
Journal Volume
13
Journal Issue
4
Pages
553-558
Date Issued
2007
Author(s)
Abstract
We evaluated an enzyme-linked immunospot assay for interferon-γ (T SPOT-TB) for rapid diagnosis of active tuberculosis (TB) in a disease-endemic area. From January to June 2005, patients whose clinical symptoms and radiographic findings were compatible with TB were recruited, and a blood sample was obtained for T SPOT-TB assay within 7 days of microbiologic studies. Sixty-five patients were studied, including 39 (60%) with active TB. Thirty-five (53.8%) patients had underlying medical conditions. Thirty-seven patients had positive cultures for Mycobacterium tuberculosis, and 11 patients had positive cultures for non-tuberculous mycobacteria. The sensitivity, specificity, positive predictive value, and negative predictive value of the T SPOT-TB assay were 87.2%, 88.5%, 91.9%, and 82.1%, respectively. The accuracy of this test in diagnosing active TB is >80%, even in an area with a high incidence of non-tuberculous mycobacterial disease.
SDGs
Other Subjects
BCG vaccine; clarithromycin; ethambutol; gamma interferon; isoniazid; rifampicin; adolescent; adult; aged; article; bacterium culture; blood sampling; child; clinical feature; controlled study; diagnostic accuracy; enzyme linked immunospot assay; female; human; major clinical study; male; microbiological examination; morbidity; Mycobacterium tuberculosis; radiography; sensitivity and specificity; treatment response; tuberculosis
Type
journal article