https://scholars.lib.ntu.edu.tw/handle/123456789/563417
Title: | Enterohaemorrhagic Escherichia coli O157: H7 Shiga-like toxin 1 is required for full pathogenicity and activation of the p38 mitogen-activated protein kinase pathway in Caenorhabditis elegans | Authors: | Chou, T.-C. HAO-CHIEH CHIU Kuo, C.-J. Wu, C.-M. Syu, W.-J. Chiu, W.-T. Chen, C.-S. |
Issue Date: | 2013 | Journal Volume: | 15 | Journal Issue: | 1 | Source: | Cellular Microbiology | Abstract: | Enterohaemorrhagic Escherichia coli (EHEC) causes life-threatening infections in humans as a consequence of the production of Shiga-like toxins. Lack of a good animal model system currently hinders in vivo study of EHEC virulence by systematic genetic methods. Here we applied the genetically tractable animal, Caenorhabditis elegans, as a surrogate host to study the virulence of EHEC as well as the host immunity to this human pathogen. Our results show that E. coli O157:H7, a serotype of EHEC, infects and kills C. elegans. Bacterial colonization and induction of the characteristic attaching and effacing (A/E) lesions in the intact intestinal epithelium of C. elegans by E. coli O157:H7 were concomitantly demonstrated in vivo. Genetic analysis indicated that the Shiga-like toxin 1 (Stx1) of E. coli O157:H7 is a virulence factor in C. elegans and is required for full toxicity. Moreover, the C. elegans p38 mitogen-activated protein kinase (MAPK) pathway, an evolutionarily conserved innate immune and stress response signalling pathway, is activated in the regulation of host susceptibility to EHEC infection in a Stx1-dependent manner. Our results validate the EHEC-C. elegans interaction as suitable for future comprehensive genetic screens for both novel bacterial and host factors involved in the pathogenesis of EHEC infection. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/563417 | ISSN: | 14625814 | DOI: | 10.1111/cmi.12030 | SDG/Keyword: | mitogen activated protein kinase p38; verotoxin 1; article; bacterial colonization; bacterial gene; bacterial immunity; bacterial virulence; Caenorhabditis elegans; colony forming unit; confocal microscopy; controlled study; death; disease model; enterohemorrhagic Escherichia coli; enzyme activation; Escherichia coli infection; Escherichia coli O157; fluorescence microscopy; in vivo study; infection sensitivity; innate immunity; intestine epithelium; nonhuman; priority journal; reverse transcription polymerase chain reaction; stress; transmission electron microscopy; Animals; Caenorhabditis elegans; Escherichia coli Infections; Escherichia coli O157; Host-Pathogen Interactions; Intestinal Mucosa; Models, Animal; p38 Mitogen-Activated Protein Kinases; Shiga Toxin 1; Survival Analysis; Virulence Factors; Animalia; Bacteria (microorganisms); Caenorhabditis elegans; Escherichia coli |
Appears in Collections: | 醫學檢驗暨生物技術學系 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.