https://scholars.lib.ntu.edu.tw/handle/123456789/564605
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wu M.-Y. | en_US |
dc.contributor.author | Lin T.-H. | en_US |
dc.contributor.author | Chiu Y.-C. | en_US |
dc.contributor.author | Liou H.-C. | en_US |
dc.contributor.author | RONG-SEN YANG | en_US |
dc.contributor.author | WEN-MEI FU | en_US |
dc.date.accessioned | 2021-06-01T07:03:20Z | - |
dc.date.available | 2021-06-01T07:03:20Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 7302312 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/564605 | - |
dc.description.abstract | 15-Lipoxygenase (15-LOX) is involved in many pathological processes. The aim of this study is to examine the role of 15-LOX in the matrix metalloproteinase (MMP) expression and inflammatory arthritis. It was found that treatment of 15-LOX downstream product of 15-(S)-HETE (15-S- hydroxyeicosatetraenoic acid) increased the mRNA and protein levels of MMP-2 in rheumatoid arthritis synovial fibroblast (RASF) derived from rheumatoid arthritis patients. The enhancement effect of 15-(S)-HETE was antagonized by the addition of LY294002 (PI3K inhibitor) and PDTC (NF-κB inhibitor). Treatment of 15-(S)-HETE increased the phosphorylation of AKT, nuclear translocation of p65 and the breakdown of IIα. TNF-α and IL-1β are the key cytokines involved in arthritis and also increase the activity of MMP-2 in RASF, which was antagonized by pretreatment with 15-LOX inhibitor PD146176 or knockdown of 15-LOX. It was also found that these two cytokines increased the expression of 15-LOX in RASF. Treatment of glucocorticoid but not NSAIDs inhibited 15-(S)-HETE-induced expression of MMP-2. In comparison with wild-type mice, adjuvant-induced arthritis and MMP-2 expression in synovial membrane were markedly inhibited in 15-LOX knockout (KO) mice. These results indicate that 15-LOX plays an important role in the disease progression of arthritis and may be involved in the inflammatory action induced by TNF-α and IL-1β. 15-LOX is thus a good target for developing drugs in the treatment of inflammatory arthritis. ? 2012 Wiley Periodicals, Inc. | - |
dc.relation.ispartof | Journal of Cellular Biochemistry | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | 15 hydroxyicosatetraenoic acid; 2 morpholino 8 phenylchromone; arachidonate 15 lipoxygenase; gelatinase A; glucocorticoid; I kappa B alpha; immunoglobulin enhancer binding protein; interleukin 1beta; matrix metalloproteinase; protein kinase B; pyrrolidine dithiocarbamate; synaptotagmin I; tumor necrosis factor alpha; animal tissue; article; fibroblast; human; human cell; mouse; nonhuman; priority journal; protein expression; protein phosphorylation; rheumatoid arthritis; rheumatoid arthritis synovial fibroblast; synovium; wild type; Animals; Arachidonate 15-Lipoxygenase; Arthritis, Experimental; Arthritis, Rheumatoid; Cells, Cultured; Chromones; Eicosapentaenoic Acid; Fibroblasts; Fluorenes; Humans; I-kappa B Kinase; Interleukin-1beta; Matrix Metalloproteinase 2; Mice; Mice, Inbred C57BL; Mice, Knockout; Morpholines; Phosphatidylinositol 3-Kinases; Phosphorylation; Proline; Proto-Oncogene Proteins c-akt; RNA Interference; RNA, Messenger; RNA, Small Interfering; Synovial Membrane; Thiocarbamates; Transcription Factor RelA; Tumor Necrosis Factor-alpha; Mus | - |
dc.title | Involvement of 15-lipoxygenase in the inflammatory arthritis | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/jcb.24098 | - |
dc.identifier.pmid | 22573549 | - |
dc.identifier.scopus | 2-s2.0-84861022552 | - |
dc.relation.pages | 2279-2289 | - |
dc.relation.journalvolume | 113 | - |
dc.relation.journalissue | 7 | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Orthopedic Surgery-NTUH | - |
crisitem.author.dept | Orthopedic Surgery | - |
crisitem.author.dept | Pharmacology | - |
crisitem.author.orcid | 0000-0002-0553-4779 | - |
crisitem.author.orcid | 0000-0003-4157-8987 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
Appears in Collections: | 藥理學科所 |
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