https://scholars.lib.ntu.edu.tw/handle/123456789/567281
標題: | YC-1 targeting of hypoxia-inducible factor-1α reduces RGC-5 cell viability and inhibits cell proliferation | 作者: | Tsui L. Fong T.-H. I-JONG WANG |
公開日期: | 2012 | 卷: | 18 | 起(迄)頁: | 1594-1603 | 來源出版物: | Molecular Vision | 摘要: | Purpose: The survival of retinal ganglion cells (RGCs) is a hallmark of many optic neurodegenerative diseases such as glaucoma. YC-1, a potential anticancer drug, is known to be able to decrease the stability and protein expression of hypoxiainducible factor (HIF)-1α that is triggered by hypoxia and related to RGC survival. We hypothesized that YC-1 may alter RGC cell viability through the down-regulation of HIF-1α. Methods: Cell viability of the RGC-5 cell line was measured with a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Flow cytometry, a LIVE/DEAD viability assay, and high-content screening (HCS) with MKI67 (K i-67) monoclonal antibodies were used to detect cell death and cellular proliferation. Results: We found that cells treated with 20 μM YC-1 for 24 h decreased the HIF-1α level in an RGC-5 cell line using immunoblotting and reduced the live cell number in an MTT assay. Results of flow cytometry and HCS demonstrated that reducing the cell proliferation of RGC-5 cells, not cell death, led to the decreased level in the MTT assay. Conclusions: Our findings demonstrate that YC-1-induced down-regulation of HIF-1α might reduce RGC cell proliferation and viability under normoxia, which implies a role of YC-1 in the neuroprotective effect for further clinical applications. ? 2012 Molecular Vision. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84863322445&partnerID=40&md5=705b18cb53e2066c668afe9eaa175d62 https://scholars.lib.ntu.edu.tw/handle/123456789/567281 |
ISSN: | 1090-0535 | SDG/關鍵字: | hypoxia inducible factor 1alpha; lificiguat; apoptosis; article; cell count; cell death; cell level; cell proliferation; cell viability; controlled study; down regulation; drug mechanism; priority journal; retina ganglion cell; Antibodies; Antineoplastic Agents; Apoptosis; Cell Line; Cell Proliferation; Cell Survival; Dose-Response Relationship, Drug; Down-Regulation; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Indazoles; Ki-67 Antigen; Retinal Ganglion Cells; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Signal Transduction |
顯示於: | 醫學系 |
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