https://scholars.lib.ntu.edu.tw/handle/123456789/568033
標題: | Ribavirin or CpG DNA sequence-modulated dendritic cells decrease the IgE level and airway inflammation | 作者: | Chiang D.-J. Ye Y.-L. Chen W.-L. Lee Y.-L. Hsu N.-Y. BOR-LUEN CHIANG |
關鍵字: | Asthma; CpG-ODN; Dendritic cells; Ribavirin | 公開日期: | 2003 | 卷: | 168 | 期: | 5 | 起(迄)頁: | 575-580 | 來源出版物: | American Journal of Respiratory and Critical Care Medicine | 摘要: | Asthma is an allergic disease that is characterized by the imbalance between Th1 and Th2 cells and by the predominant Th2-type immune response. In this study, we investigated the application of dendritic cell (DCs)-based immunotherapy in modulating the immune response of allergic diseases. DCs incubated with ovalbumin (OVA), OVA plus ribavirin, OVA plus CpG-oligodeoxynucleotides (ODN 1826), or OVA plus non-CpG-ODN (ODN 1745) for 48 hours were injected intravenously into four corresponding groups of BALB/c mice. All of the mice were then immunized with OVA intraperitoneally 7 days later to establish an animal model of asthma. Serum levels of OVA antibody, airway hyperresponsivness, cell composition and cytokine levels in the bronchoalveolar lavage fluid, and cytokine profiles of spleen cells were analyzed. The data showed that ribavirin and ODN 1826 increased interleukin-12 synthesis and inhibited interleukin-10 production. ODN 1826 could also enhance the expression of B7.1, B7.2, major histocompatibility complex I, and major histocompatibility complex II molecules. Furthermore, the DCs modulated by ribavirin and ODN 1826 could downregulate the Th2-type immune response in vivo and could alleviate airway inflammation. This study elucidated the effect of ribavirin and CpG-ODN on DCs and demonstrated that in vitro modulated DCs might be a potential therapeutic approach for asthma. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042877077&doi=10.1164%2frccm.2205005&partnerID=40&md5=1ab38a08a8d83ab9ddd05c4899739169 https://scholars.lib.ntu.edu.tw/handle/123456789/568033 |
ISSN: | 1073-449X | DOI: | 10.1164/rccm.2205005 | SDG/關鍵字: | immunoglobulin; oligodeoxynucleotide; ovalbumin; recombinant vaccine; ribavirin; acute phase response; adoptive immunotherapy; airway conductance; allergic asthma; animal cell; animal experiment; antibody blood level; article; cell composition; controlled study; CpG island; cytokine production; dendritic cell; DNA sequence; down regulation; experimental model; female; immunoglobulin blood level; immunomodulation; immunoprophylaxis; injection site; lung lavage; major histocompatibility complex; mouse; nonhuman; priority journal; spleen cell; Th2 cell; Adjuvants, Immunologic; Animals; Antiviral Agents; Asthma; Base Sequence; Dendritic Cells; Disease Models, Animal; Female; Immunoglobulin E; Immunotherapy; Mice; Mice, Inbred BALB C; Oligodeoxyribonucleotides; Ribavirin |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。