https://scholars.lib.ntu.edu.tw/handle/123456789/568218
Title: | ARL4, an ARF-like protein that is developmentally regulated and localized to nuclei and nucleoli | Authors: | Lin C.-Y. PEI-HSIN HUANG Liao W.-L. Cheng H.-J. Huang C.-F. Kuo J.-C. Patton W.A. Massenburg D. Moss J. Fang-Jen Scott Lee |
Issue Date: | 2000 | Journal Volume: | 275 | Journal Issue: | 48 | Start page/Pages: | 37815-37823 | Source: | Journal of Biological Chemistry | Abstract: | ADP-ribosylation factors (ARFs) are highly conserved ~20-kDa guanine nucleotide-binding proteins that participate in both exocytic and endocytic vesicular transport pathways via mechanisms that are only partially understood. Although several ARF-like proteins (ARLs) are known, their biological functions remain unclear. To characterize its molecular properties, we cloned mouse and human ARL4 (mARL4 and hARL4) cDNA. The appearance of mouse ARL4 mRNA during embryonic development coincided temporally with the sequential formation of somites and the establishment of brain compartmentation. Using ARL4-specific antibody for immunofluorescence microscopy, we observed that endogenous mARL4 in cultured Sertoli and neuroblastoma cells was mainly concentrated in nuclei. When expressed in COS7 cells, ARL4-T34N mutant, predicted to exist with GDP bound, was concentrated in nucleoli. Yeast two-hybrid screening and in vitro protein-interaction assays showed that hARL4 interacted with importin-α through its C-terminal NLS region and that the interaction was not nucleotide-dependent. Like ARL2 and -3, recombinant hARL4 did not enhance cholera toxin-catalyzed auto-ADP-ribosylation. Its binding of guanosine 5'-O-(thiotriphosphate) was modified by phospholipid and detergent, and the N terminus of hARL4, like that of ARF, was myristoylated. Our findings suggest that ARL4, with its distinctive nuclear/nucleolar localization and pattern of developmental expression, may play a unique role(s) in neurogenesis and somitogenesis during embryonic development and in the early stages of spermatogenesis in adults. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034535510&doi=10.1074%2fjbc.M002470200&partnerID=40&md5=fb53470e1c4d0215754980ce288d2692 https://scholars.lib.ntu.edu.tw/handle/123456789/568218 |
ISSN: | 0021-9258 | DOI: | 10.1074/jbc.M002470200 | SDG/Keyword: | adenosine diphosphate ribosylation factor; carrier protein; adenosine diphosphate ribosylation; animal cell; article; brain development; embryo development; molecular cloning; nerve cell; nonhuman; nucleolus; priority journal; protein localization; protein protein interaction; spermatogenesis; ADP-Ribosylation Factors; Amino Acid Sequence; Animals; Base Sequence; Cell Nucleolus; Cell Nucleus; DNA Primers; DNA, Complementary; Gene Expression Regulation, Developmental; Humans; Mice; Two-Hybrid System Techniques; Animalia |
Appears in Collections: | 分子醫學研究所 |
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