https://scholars.lib.ntu.edu.tw/handle/123456789/568295
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lin G.-Y. | en_US |
dc.contributor.author | Wu Y.-L. | en_US |
dc.contributor.author | Wang C.-S. | en_US |
dc.contributor.author | Ko C.-Y. | en_US |
dc.contributor.author | Chen C.-H. | en_US |
dc.contributor.author | PEI-JER CHEN | en_US |
dc.contributor.author | Peng P.-H. | en_US |
dc.contributor.author | Hsu C.-W. | en_US |
dc.date.accessioned | 2021-07-03T03:33:24Z | - |
dc.date.available | 2021-07-03T03:33:24Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 1743-422X | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086686454&doi=10.1186%2fs12985-020-01355-5&partnerID=40&md5=cf0d045f355dc0e27aa4fd34b96ea458 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/568295 | - |
dc.description.abstract | Background: Hepatitis D virus (HDV) infection is a major global health issue around the world. There are approximately 15-20 million individuals infected with HDV worldwide. HDV infection usually causes increased mortality compared with infection with hepatitis B virus (HBV) alone. However, testing for the detection of HDV is not widely available in Taiwan. Therefore, the General Biologicals Corporation (GB) HDV Ab kit was developed for detecting anti-HDV antibodies. Methods: A total of 913 serum and 462 EDTA-treated plasma samples were obtained from HBsAg-positive individuals in three hospitals in Taiwan from June 2014 to November 2017. We used three commercially available ELISA kits, DiaPro HDV Ab, DiaSorin ETI-AB-DELTAK-2 and GB HDV Ab, which were utilized strictly according to the instructions of the manufacturers. Results: A comparative study of the results from the GB HDV Ab kit and the other commercial ELISA kits (DiaPro and DiaSorin) was performed to determine their efficacy for anti-HDV detection. The results indicated that the sensitivity of the GB HDV Ab kit for serum and EDTA samples was 100% compared to that of the DiaPro and DiaSorin kits, whereas the specificity for serum and EDTA samples was 99.3 and 98.1%, respectively. In addition, the overall agreement of the results of the GB HDV Ab kit for the serum and EDTA samples was 99.3 and 98.3%, respectively. It is worth noting that the performance of the GB HDV Ab kit was not affected by interference from triglyceride, bilirubin, hemoglobin, or human anti-mouse antibody. The limit of detection of the GB HDV Ab kit is approximately 100-fold lower than that of the other two commercial kits. Conclusions: The GB HDV Ab kit, which presented equivalent sensitivity and specificity compared to both certified anti-HDV kits, would be a suitable kit for HDV diagnosis in Taiwan. ? 2020 The Author(s). | - |
dc.publisher | BioMed Central Ltd. | - |
dc.relation.ispartof | Virology Journal | - |
dc.subject | ELISA; HDV; Kit evaluation; Sensitivity; Specificity | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | bilirubin; hemoglobin; hepatitis B surface antigen; triacylglycerol; virus antibody; hepatitis antibody; antibody detection; Article; comparative effectiveness; controlled study; enzyme linked immunosorbent assay; Hepatitis delta virus; human; limit of detection; major clinical study; sensitivity and specificity; serum; Taiwan; blood; comparative study; delta agent hepatitis; diagnostic kit; enzyme linked immunosorbent assay; Hepatitis delta virus; immunology; Enzyme-Linked Immunosorbent Assay; Hepatitis Antibodies; Hepatitis D; Hepatitis Delta Virus; Humans; Limit of Detection; Reagent Kits, Diagnostic; Sensitivity and Specificity; Taiwan | - |
dc.title | Performance of commercially available anti-HDV enzyme-linked immunosorbent assays in Taiwan | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1186/s12985-020-01355-5 | - |
dc.identifier.pmid | 32546164 | - |
dc.identifier.scopus | 2-s2.0-85086686454 | - |
dc.relation.pages | 76 | - |
dc.relation.journalvolume | 17 | - |
dc.relation.journalissue | 1 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.orcid | 0000-0001-8316-3785 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 臨床醫學研究所 |
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