https://scholars.lib.ntu.edu.tw/handle/123456789/568351
標題: | Intracellular hepatitis B virus increases hepatic cholesterol deposition in alcoholic fatty liver via hepatitis B core protein | 作者: | Wang Y. Wu T. Hu D. Weng X. Wang X. PEI-JER CHEN Luo X. Wang H. Ning Q. |
關鍵字: | Alcoholic liver disease; Cholesterol; Hepatitis b virus | 公開日期: | 2018 | 出版社: | American Society for Biochemistry and Molecular Biology Inc. | 卷: | 59 | 期: | 1 | 起(迄)頁: | 58-68 | 來源出版物: | Journal of Lipid Research | 摘要: | Hepatitis B virus (HBV) infection is a prevalent infectious disease with serious outcomes like chronic and acute hepatitis, cirrhosis, and hepatocellular carcinoma. However, the metabolic alteration by HBV is rarely taken into consideration. With the high prevalence of alcohol consumption and chronic HBV infection, their overlap is assumed to be an increasing latent hazard; although the extent has not been calculated. Moreover, the impact of chronic alcohol consumption combined with HBV on cholesterol metabolism is unknown. Six-week-old male FVB/Ncrl mice were hydrodynamically injected with a pGEM-4Z-1.3HBV vector and then fed an ethanol diet for 6 weeks. Serum biomarkers and liver histology, liver cholesterol levels, and cholesterol metabolismrelated molecules were measured. In vitro assays with HBx, hepatitis B surface (HBs), or hepatitis B core (HBc) protein expression in HepG2 cells costimulated with ethanol were conducted to assess the cholesterol metabolism. HBV expression synergistically increased cholesterol deposition in the setting of alcoholic fatty liver. The increase of intrahepatic cholesterol was due to metabolic alteration in cholesterol metabolism, including increased cholesterol synthesis, decreased cholesterol degradation, and impaired cholesterol uptake. Overexpression of HBV component HBc, but not HBs or HBx, selectively promoted the hepatocellular cholesterol level. Copyright ? 2018 by the American Society for Biochemistry and Molecular Biology, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85039904348&doi=10.1194%2fjlr.M079533&partnerID=40&md5=cafba2e7a5b47ac0d079eda7378db677 https://scholars.lib.ntu.edu.tw/handle/123456789/568351 |
ISSN: | 0022-2275 | DOI: | 10.1194/jlr.M079533 | SDG/關鍵字: | biological marker; cholesterol; core protein; hepatitis B core protein; hepatitis B surface antigen; unclassified drug; virus protein V; virus vector; cholesterol; core protein; alcohol consumption; alcoholic fatty liver; animal experiment; animal model; animal tissue; Article; bioassay; cholesterol liver level; cholesterol metabolism; cholesterol synthesis; cholesterol transport; controlled study; degradation kinetics; Hep-G2 cell line; Hepatitis B virus; in vitro study; in vivo study; lipid storage; liver histology; male; mouse; nonhuman; priority journal; protein expression; risk assessment; upregulation; alcoholic fatty liver; animal; disease model; Hepatitis B virus; human; inbred mouse strain; liver; metabolism; tumor cell culture; virology; Animals; Cholesterol; Disease Models, Animal; Fatty Liver, Alcoholic; Hep G2 Cells; Hepatitis B virus; Humans; Liver; Male; Mice; Mice, Inbred Strains; Tumor Cells, Cultured; Viral Core Proteins |
顯示於: | 臨床醫學研究所 |
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