https://scholars.lib.ntu.edu.tw/handle/123456789/568789
Title: | Single nucleotide polymorphisms in intron 2 of the human interleukin-1 receptor antagonist (IL-1Ra) gene: Further definition of the IL-1β and IL-1Ra polymorphisms in North American Caucasians and Taiwanese Chinese | Authors: | LI-HUI TSENG PEI-JER CHEN Lin M.-T. Shau W.-Y. Chaung S.-M. Martin P.J. Hansen J.A. |
Issue Date: | 2001 | Journal Volume: | 57 | Journal Issue: | 4 | Start page/Pages: | 318-324 | Source: | Tissue Antigens | Abstract: | Previous studies have suggested that a variable number tandem repeat (VNTR) polymorphism in the second intron of the interleukin-1 receptor antagonist (IL-1Ra) gene and the single nucleotide polymorphisms at positions -511 and +3954 of the IL-1β gene might be associated with increased risks of chronic inflammatory diseases, autoimmune diseases and gastric cancer. In the present study, IL-1β and IL-1Ra genotypes were analyzed among Asians in Taiwan and Caucasians in North America. We identified a novel polymorphism with 3 nucleotide substitutions in the IL-1Ra VNTR 2-repeat allele. One of the substitutions corresponds with the fourth 3′ end nucleotide of the reverse primer that is often used for analysis of the IL-1Ra-associated VNTR locus. Mismatching between this primer and the 2-repeat allele can cause misleading amplification results when stringent conditions are used for annealing. The estimated haplotype frequencies of the variant IL-1 genes were significantly different between Taiwanese and Caucasians. The frequency of the pro-inflammatory IL-1Ra 2-repeat allele was significantly lower in Taiwanese than in Caucasians. In contrast, the frequencies of the pro-inflammatory IL-1β -511T allele and +3954C allele were significantly higher among Taiwanese compared with Caucasians. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034993606&doi=10.1034%2fj.1399-0039.2001.057004318.x&partnerID=40&md5=23fd28fbc80c6a17648bce8e3adc6ac4 https://scholars.lib.ntu.edu.tw/handle/123456789/568789 |
ISSN: | 0001-2815 | DOI: | 10.1034/j.1399-0039.2001.057004318.x | SDG/Keyword: | interleukin 1 receptor blocking agent; interleukin 1beta; article; Caucasian; Chinese; gene; gene amplification; gene frequency; gene locus; gene sequence; genotype; haplotype; human; intron; molecular cloning; nucleotide repeat; nucleotide sequence; priority journal; sequence analysis; single nucleotide polymorphism; tandem repeat; Asian Continental Ancestry Group; Base Pair Mismatch; DNA Primers; European Continental Ancestry Group; Gene Frequency; Genotype; Haplotypes; Histocompatibility Testing; Humans; Interleukin 1 Receptor Antagonist Protein; Interleukin-1; Introns; Minisatellite Repeats; North America; Polymorphism, Single Nucleotide; Receptors, Interleukin-1; Sialoglycoproteins; Taiwan |
Appears in Collections: | 臨床醫學研究所 |
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