https://scholars.lib.ntu.edu.tw/handle/123456789/569209
標題: | Immunohistochemical analysis of Th1/Th2 cytokine profiles and androgen receptor expression in the pathogenesis of nifedipine-induced gingival overgrowth | 作者: | Huang W.-T. Lu H.-K. Chou H.-H. YEN-PING KUO |
關鍵字: | Androgen receptor; Gingival overgrowth; Nifedipine; Periodontitis | 公開日期: | 2003 | 出版社: | Blackwell Munksgaard | 卷: | 38 | 期: | 4 | 起(迄)頁: | 422-427 | 來源出版物: | Journal of Periodontal Research | 摘要: | Background: Numerous studies have demonstrated that gingival overgrowth may be associated with androgen and cytokine expression in tissues. Objectives: The aim of this study was to compare the expression of androgen receptor-presenting cells (AR+ cells) and Th1/Th2 cytokine [Th1: interleukin (IL)-2, interferon-γ (IFN-γ); Th2: IL-4, IL-10, IL-13] expression cells in tissue sections of patients with gingival overgrowth. Materials and methods: Tissue samples were collected from patients with healthy periodontium (H group), adult periodontitis (P group), surgically extracted teeth (S group), and nifedipine-induced gingival overgrowth (NIGO group). The clinical periodontal parameters of pocket depth (PD), bleeding on probing (BOP), and plaque control record (PCR) were measured around selected sample teeth. Gingival biopsies were further processed by immunohistochemical staining method. The expressions of cells positive for AR, IL-2, IFN-γ, IL-4, IL-10, and IL-13 were counted by predetermined semiquantitative methods. Results: Our results indicated that AR, IL-2, IFN-γ, IL-4, IL-10, and IL-13 were intensively expressed in the nuclei of inflammatory cells and fibroblasts of gingival connective tissue. Stronger expressions of AR, IL-2, and IFN-γ were found in the NIGO group. The AR+ cells/0.01 mm2 in gingival fibroblasts were significantly higher in the NIGO group (80.2 ± 10.7) than those of the periodontitis group (52.5 ± 11.8) and control group (37.4 ± 11.3) (P < 0.05). The cytokine expression of the NIGO group showed a trend towards Th1-type expression (IL-2; P = 0.0001). In the surgically extracted tooth group, a stronger expression of Th2-type cytokine (IL-4, Il-10, IL-13; P < 0.05) was found in inflammatory cells. In a comparison of the IL-2/IL-4-labeled cell ratio of the four groups, a descending sequence was discovered as NIGO group (0.92 ± 0.97) > H group (0.81 ± 0.61) > P group (0.77 ± 0.82) > S group (0.58 ± 1.77). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141671854&doi=10.1034%2fj.1600-0765.2003.00672.x&partnerID=40&md5=75ef95fc516734e9132f420740341bfd https://scholars.lib.ntu.edu.tw/handle/123456789/569209 |
ISSN: | 0022-3484 | DOI: | 10.1034/j.1600-0765.2003.00672.x | SDG/關鍵字: | androgen receptor; cytokine; gamma interferon; interleukin 10; interleukin 13; interleukin 2; interleukin 4; nifedipine; androgen receptor; calcium channel blocking agent; gamma interferon; interleukin 10; interleukin 13; interleukin 2; interleukin 4; interleukin derivative; adult; aged; amino acid sequence; article; cell activity; cell labeling; cell nucleus; cellular distribution; clinical article; connective tissue; controlled study; cytokine production; disease predisposition; female; fibroblast; gingiva bleeding; gingiva hyperplasia; gingival biopsy; human; human tissue; immunohistochemistry; inflammatory cell; male; medical parameters; medical record; pathogenesis; periodontics; periodontitis; periodontium; protein determination; protein expression; quantitative analysis; T lymphocyte; Th1 cell; Th2 cell; tissue section; tooth extraction; tooth plaque; chemically induced disorder; comparative study; gingiva overgrowth; metabolism; middle aged; Th1 cell; Th2 cell; Adult; Calcium Channel Blockers; Cell Nucleus; Connective Tissue; Female; Fibroblasts; Gingival Overgrowth; Humans; Immunohistochemistry; Interferon Type II; Interleukin-10; Interleukin-13; Interleukin-2; Interleukin-4; Interleukins; Male; Middle Aged; Nifedipine; Periodontal Index; Periodontitis; Receptors, Androgen; Th1 Cells; Th2 Cells |
顯示於: | 牙醫學系 |
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