https://scholars.lib.ntu.edu.tw/handle/123456789/569239
標題: | Collagen Biosynthesis in Human Oral Submucous Fibrosis Fibroblast Cultures | 作者: | YEN-PING KUO HSIN-MING CHEN Hahn L.J. Hsieh C.C. CHUN-PIN CHIANG |
公開日期: | 1995 | 卷: | 74 | 期: | 11 | 起(迄)頁: | 1783-1788 | 來源出版物: | Journal of Dental Research | 摘要: | To investigate the mechanism of collagen accumulation in oral submucous fibrosis (OSF) tissues, we examined the biosynthesis of collagen in fibroblast cultures established from OSF lesions. Fibroblasts obtained from four of ten OSF specimens showed more than a 1.5-fold increase in the production of collagens compared with fibroblasts from age-, sex-, and passage-matched normal controls (p < 0.05). When the relative amounts of collagen synthesis were estimated by SDS polyacrylamide gel electrophoresis, it was found that both OSF and control cells produced about 85% type I collagen and 15% type III collagen. The ratio of α1(I) to a2(I) chains was about 3:1 in OSF cells instead of the 2:1 expected for type I collagen. The excess al(I) chains could mean that collagen type I trimer was synthesized by the fibroblasts. These findings suggest that collagen overproduction and a reduced degradation of the structure-stable collagen type I trimer synthesized by OSF fibroblasts might contribute to the accumulation of collagen in OSF lesions in vivo. The mechanism(s) of increased procollagen production were analyzed by Northern blot, slot blot, and Southern blot. The OSF fibroblast strains with elevated collagen production also contained higher-than-normal levels of procollagen mRNA, and the ratios of α1(I), a2(I), and α1(III) procollagen mRNAs were compatible with the results of corresponding procollagen a chains. The gene copy number of proa2(I) collagen gene in OSF fibroblasts was about 1.05. No gene amplification was found. These results indicate that expression of these procollagen genes in cultured fibroblasts is regulated at the transcriptional level. ? 1995, SAGE Publications. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029401138&doi=10.1177%2f00220345950740111101&partnerID=40&md5=fab7061e612e7abeded15940e8f10562 https://scholars.lib.ntu.edu.tw/handle/123456789/569239 |
ISSN: | 0022-0345 | DOI: | 10.1177/00220345950740111101 | SDG/關鍵字: | collagen; messenger RNA; procollagen; article; biosynthesis; case control study; cell culture; fibroblast; gene expression; genetics; human; immunoblotting; male; metabolism; mouth disease; mouth mucosa; Case-Control Studies; Cells, Cultured; Collagen; Fibroblasts; Gene Expression; Humans; Immunoblotting; Male; Mouth Mucosa; Oral Submucous Fibrosis; Procollagen; RNA, Messenger |
顯示於: | 牙醫學系 |
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