https://scholars.lib.ntu.edu.tw/handle/123456789/569264
Title: | IL-1β induced IL-8 and uPA expression/production of dental pulp cells: Role of TAK1 and MEK/ERK signaling | Authors: | Lin S.-I. LI-DEH LIN HSIAO-HUA CHANG Chang M.-C. YIN-LIN WANG Pan Y.-H. Huang G.-F. Lin H.-J. JIIANG-HUEI JENG |
Issue Date: | 2018 | Publisher: | Elsevier B.V. | Journal Volume: | 117 | Journal Issue: | 8 | Start page/Pages: | 697-704 | Source: | Journal of the Formosan Medical Association | Abstract: | Background/purpose: Interleukin 1 beta (IL-1β) is a pro-inflammatory cytokine involved in the inflammatory processes of dental pulp. IL-8 and urokinase plasminogen activator (uPA) are two inflammatory mediators. However, the role of transforming growth factor beta-activated kinase-1 (TAK1) and mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling pathways in responsible for the effects of IL-1β on IL-8 and uPA expression/secretion of dental pulp cells are not clear. Methods: Human dental pulp cells were exposed to IL-1β with/without pretreatment with 5z-7-oxozeaneaeol (a TAK1 inhibitor) or U0126 (a MEK/ERK inhibitor). TAK1 activation was determined by immunofluorescent staining. The protein expression of IL-8 was tested by western blot. The expression of IL-8 and uPA mRNA was studied by reverse transcriptase-polymerase chain reaction (RT-PCR). The secretion of IL-8 and uPA was measured by enzyme-linked immunosorbent assay. Results: Exposure of dental pulp cells to IL-1β (0.1–10 ng/ml) stimulated IL-8 and uPA expression. IL-1β also induced IL-8 and uPA secretion of dental pulp cells. IL-1β stimulated p-TAK1 activation of pulp cells. Pretreatment and co-incubation of pulp cells by 5z-7oxozeaenol (1 and 2.5 μM) and U0126 (10 and 20 μM) prevented the IL-1β-induced IL-8 and uPA expression. 5z-7oxozeaenol and U0126 also attenuated the IL-1β-induced IL-8 and uPA secretion. Conclusion: IL-1β is important in the pathogenesis of pulpal inflammatory diseases and repair via stimulation of IL-8 and uPA expression and secretion. These events are associated with TAK1 and MEK/ERK signaling. Blocking of TAK1 and MEK/ERK signaling has potential to control inflammation of dental pulp. ? 2018 |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046118294&doi=10.1016%2fj.jfma.2018.04.003&partnerID=40&md5=0f5bb6ceaa0805c694472d2f372acc43 https://scholars.lib.ntu.edu.tw/handle/123456789/569264 |
ISSN: | 0929-6646 | DOI: | 10.1016/j.jfma.2018.04.003 | SDG/Keyword: | 1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene; 5z 7 oxozeaneaeol; interleukin 1beta; interleukin 8; messenger RNA; mitogen activated protein kinase; mitogen activated protein kinase kinase; protein serine threonine kinase inhibitor; transforming growth factor beta activated kinase 1; unclassified drug; urokinase; 1,3 butadiene; 5-7-oxo-zeaenol; acetyl-lysyl-prolyl-seryl-seryl-prolyl-prolyl-glutamyl-glutamic acid amide; enzyme inhibitor; IL8 protein, human; interleukin 1beta; interleukin 8; MAP kinase kinase kinase 7; mitogen activated protein kinase kinase kinase; nitrile; peptide fragment; urokinase; zearalenone; Article; cellular secretion; enzyme linked immunospot assay; human; human cell; human tissue; immunofluorescence test; protein expression; protein phosphorylation; reverse transcription polymerase chain reaction; signal transduction; tooth pulp; analogs and derivatives; cell culture; cytology; drug effect; epithelium cell; MAPK signaling; metabolism; tooth pulp; Butadienes; Cells, Cultured; Dental Pulp; Enzyme Inhibitors; Epithelial Cells; Humans; Interleukin-1beta; Interleukin-8; MAP Kinase Kinase Kinases; MAP Kinase Signaling System; Nitriles; Peptide Fragments; Urokinase-Type Plasminogen Activator; Zearalenone |
Appears in Collections: | 牙醫學系 |
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