https://scholars.lib.ntu.edu.tw/handle/123456789/569633
標題: | Arecoline induced cell cycle arrest, apoptosis, and cytotoxicity to human endothelial cells | 作者: | Tseng S.-K. Chang M.-C. Su C.-Y. Chi L.-Y. JENNY ZWEI-CHIENG CHANG WAN-YU TSENG Yeung S.-Y. Hsu M.-L. JIIANG-HUEI JENG |
公開日期: | 2012 | 卷: | 16 | 期: | 4 | 起(迄)頁: | 1267-1273 | 來源出版物: | Clinical Oral Investigations | 摘要: | Betel quid (BQ) chewing is a common oral habit in South Asia and Taiwan. BQ consumption may increase the risk of oral squamous cell carcinoma (OSCC), oral submucous fibrosis (OSF), and periodontitis as well as systemic diseases (atherosclerosis, hypertension, etc.). However, little is known about the toxic effect of BQ components on endothelial cells that play important roles for angiogenesis, carcinogenesis, tissue fibrosis, and cardiovascular diseases. EAhy 926 (EAHY) endothelial cells were exposed to arecoline, a major BQ alkaloid, for various time periods. Cytotoxicity was estimated by 3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay. The cell cycle distribution of EAHY cells residing in sub-G0/G1, G0/G1, S-, and G2/M phases was analyzed by propidium iodide staining of cellular DNA content and flow cytometry. Some EAHY cells retracted, became round-shaped in appearance, and even detached from the culture plate after exposure to higher concentrations of arecoline (> 0. 4 mM). At concentrations of 0. 4 and 0. 8 mM, arecoline induced significant cytotoxicity to EAHY cells. At similar concentrations, arecoline induced G2/M cell cycle arrest and increased sub-G0/G1 population, a hallmark of apoptosis. Interestingly, prolonged exposure to arecoline (0. 1 mM) for 12 and 21 days significantly suppressed the proliferation of EAHY cells, whereas EAHY cells showed adaptation and survived when exposed to 0. 05 mM arecoline. These results suggest that BQ components may contribute to the pathogenesis of OSF and BQ chewing-related cardiovascular diseases via toxicity to oral or systemic endothelial cells, leading to impairment of vascular function. During BQ chewing, endothelial damage may be induced by areca nut components and associate with the pathogenesis of OSF, periodontitis, and cardiovascular diseases. ? 2011 Springer-Verlag. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864282117&doi=10.1007%2fs00784-011-0604-1&partnerID=40&md5=c1341663de73e710bae0270bed676512 https://scholars.lib.ntu.edu.tw/handle/123456789/569633 |
ISSN: | 1432-6981 | DOI: | 10.1007/s00784-011-0604-1 | SDG/關鍵字: | arecoline; cholinergic receptor stimulating agent; coloring agent; diagnostic agent; DNA; propidium iodide; tetrazolium; thiazole derivative; thiazolyl blue; apoptosis; article; cell adhesion; cell cycle; cell cycle G0 phase; cell cycle G1 phase; cell line; cell proliferation; cell shape; cell survival; culture technique; cytology; drug effect; endothelium cell; flow cytometry; human; interphase; time; vascular endothelium; Apoptosis; Arecoline; Cell Adhesion; Cell Culture Techniques; Cell Cycle; Cell Line; Cell Proliferation; Cell Shape; Cell Survival; Cholinergic Agonists; Coloring Agents; DNA; Endothelial Cells; Endothelium, Vascular; Flow Cytometry; G0 Phase; G1 Phase; Humans; Interphase; Propidium; Tetrazolium Salts; Thiazoles; Time Factors |
顯示於: | 臨床牙醫學研究所 |
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