Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Medicine / 醫學院
  3. School of Dentistry / 牙醫專業學院
  4. Clinical Dentistry / 臨床牙醫學研究所
  5. Inhibition of periodontitis induction using a stimuli-responsive hydrogel carrying naringin
 
  • Details

Inhibition of periodontitis induction using a stimuli-responsive hydrogel carrying naringin

Journal
Journal of Periodontology
Journal Volume
88
Journal Issue
2
Pages
190-196
Date Issued
2017
Author(s)
PO-CHUN CHANG  
Chao Y.-C.
Hsiao M.-H.
Chou H.-S.
Jheng Y.-H.
Yu X.-H.
Lee N.
Yang C.
Liu D.-M.
DOI
10.1902/jop.2016.160189
URI
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85011954119&doi=10.1902%2fjop.2016.160189&partnerID=40&md5=c6b11a3bd6858108b7dd4e205615d2dd
https://scholars.lib.ntu.edu.tw/handle/123456789/570571
Abstract
Background: Developing a drug carrier with favorable handling characteristics that can respond to environmental changes after inflammation, such as pH changes, may be beneficial for treating periodontitis. This study aims to investigate the preclinical feasibility of using naringin, a naturally derived polymethoxylated flavonoid compound with antiinflammatory properties, to inhibit periodontitis induction via a thermogelling and pH-responsive injectable hydrogel. Methods: The hydrogel was made of amphipathic carboxymethyl-hexanoyl chitosan (CHC), β-glycerol phosphate (β-GP), and glycerol. Thermogelling and pH-responsive characteristics of the hydrogel, as well as cell viability after treatment with the hydrogel containing naringin, were evaluated in vitro. Hydrogel was subgingivally delivered when experimental periodontitis was induced in vivo, and therapeutic effect was evaluated with microcomputed tomography imaging, histology, and expression of inflammation-associated genes, including toll-like receptor (TLR)2, the receptor for advanced glycation end products (RAGE), myeloid differentiation primary response gene-88, and tumor necrosis factor (TNF)-α. Results: The hydrogel was consistently fluidic at 4°C but rapidly gelled at 37°C. Release of naringin was faster at pH 5.5 to 6.5, and viability was significantly promoted by treatment with 0.85% naringin. Naringin-carrying CHC-β-GP-glycerol hydrogel sites showed significantly reduced periodontal bone loss (P <0.05) and inflammatory infiltration (P <0.01) as well as significantly downregulated TLR2 (P <0.05), RAGE (P <0.01), and TNF-α (P <0.05) relative to the sites with experimental periodontitis alone. Conclusion: Naringin-carrying CHC-β-GP-glycerol colloidal hydrogel can be used to inhibit induction of experimental periodontitis with favorable handling and inflammation-responsive characteristics. ? 2017 American Academy of Periodontology. All rights reserved.
Subjects
Chitosan; Drug delivery systems; Flavonoids; Inflammation; Periodontitis
SDGs

[SDGs]SDG3

Other Subjects
aurantiin; drug carrier; flavanone derivative; polyethylene glycol dimethacrylate hydrogel; animal; C57BL mouse; cell culture; cell survival; chemistry; cytology; diagnostic imaging; disease model; human; male; micro-computed tomography; mouse; periodontal ligament; periodontitis; Animals; Cell Survival; Cells, Cultured; Disease Models, Animal; Drug Carriers; Flavanones; Humans; Hydrogel, Polyethylene Glycol Dimethacrylate; Male; Mice; Mice, Inbred C57BL; Periodontal Ligament; Periodontitis; X-Ray Microtomography
Publisher
American Academy of Periodontology
Type
journal article

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science