https://scholars.lib.ntu.edu.tw/handle/123456789/570654
Title: | Arecoline inhibits endothelial cell growth and migration and the attachment to mononuclear cells | Authors: | Tseng S.-K. Chang M.-C. Hsu M.-L. Su C.-Y. Chi L.-Y. Lan W.-C. JIIANG-HUEI JENG |
Keywords: | arecoline; betel quid; cardiovascular diseases; endothelial cells; oral cancer; oral submucous fibrosis | Issue Date: | 2014 | Publisher: | Association for Dental Sciences of the Republic of China | Journal Volume: | 9 | Journal Issue: | 3 | Start page/Pages: | 258-264 | Source: | Journal of Dental Sciences | Abstract: | Background/purpose Betel quid (BQ) chewing is a popular habit in South-Asian and Southeast Asian countries, and Taiwan. BQ chewing can cause oral cancer and oral submucous fibrosis, and increases the risk of cardiovascular diseases. However, how BQ chewing affects endothelial cells and is involved in cardiovascular diseases and vascular changes is not fully understood. The effects of arecoline, a component of BQ, on the growth and migration of endothelial cells (EAhy 926), and their adherence by U937 cells were investigated. Materials and methods EAhy926 endothelial cells were cultured and exposed to various concentrations of arecoline for 24 hours. Morphological changes were observed using phase-contrast microscopy. Cytotoxic effects were analyzed using a 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. A wound closure assay was used to evaluate the cellular migration of EAhy926 cells. The attachment of 2′,7′-bis-(2-carboxyethyl)-5-(6)- carboxyfluorescein-labeled U937 cells to EAhy926 endothelial cells that were pretreated with various concentrations of arecoline was further studied. Results The addition of arecoline at concentrations >0.4 mM significantly decreased cellular viability. EAhy endothelial cells showed marked morphological changes, and cellular migration decreased after 24 hours and 48 hours of exposure to arecoline. The number of U937 cells attached to EAhy 926 cells increased when endothelial cells were pretreated by arecoline. Conclusion Arecoline impaired vascular endothelial cells by inhibiting their growth and migration and their adhesion to U937 mononuclear cells. These results reveal that arecoline may contribute to the pathogenesis of oral submucous fibrosis and cardiovascular diseases by affecting endothelial cell function in BQ chewers. ? 2012, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924021814&doi=10.1016%2fj.jds.2012.12.015&partnerID=40&md5=4f128f7f72e13d65eee173533ba07624 https://scholars.lib.ntu.edu.tw/handle/123456789/570654 |
ISSN: | 1991-7902 | DOI: | 10.1016/j.jds.2012.12.015 |
Appears in Collections: | 臨床牙醫學研究所 |
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