|Title:||Dongshaea marina gen. Nov., sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production||Authors:||Huang S.-P
CHIH-LIN WEI et al.
|Keywords:||carbohydrate; carbon dioxide; fatty acid; glucose; phosphatidylethanolamine; RNA 16S; ubiquinone; water; bacterial DNA; phospholipid; sea water; ubiquinone 8; Aeromonadaceae; anaerobic bacterium; anaerobic growth; Article; bacterial cell; bacterium isolate; chemotaxonomy; cladistics; DNA base composition; Dongshaea marina; fermentation; Gammaproteobacteria; gene sequence; high performance liquid chromatography; lagoon; marine bacterium; nonhuman; Oceanimonas; Oceanisphaera; phylogeny; priority journal; sample; sediment; Zobellella; bacterium identification; chemistry; classification; DNA sequence; gas; genetics; isolation and purification; metabolism; microbiology; phylogeny; Taiwan; Aeromonadaceae; Anaerobiosis; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Fermentation; Gases; Geologic Sediments; Glucose; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Taiwan; Ubiquinone||Issue Date:||2019||Journal Volume:||69||Journal Issue:||11||Start page/Pages:||3318-3325||Source:||International Journal of Systematic and Evolutionary Microbiology||Abstract:||
Two isolates of heterotrophic, facultatively anaerobic, marine bacteria, designated DM1 and DM2T, were recovered from a lagoon sediment sample of Dongsha Island, Taiwan. Cells were Gram-reaction-negative rods. Nearly all of the cells were non-motile and non-flagellated during the late exponential to early stationary phase of growth, while a few of the cells exhibited motility with monotrichous flagellation. The two isolates required NaCl for growth and grew optimally at about 30 C, 2–3 % NaCl and pH 7–8. They grew aerobically and could achieve anaerobic growth by fermenting D-glucose or other carbohydrates with production of acids and the gases, including CO2 and H2. Ubiquinone Q-8 was the only respiratory quinone. Cellular fatty acids were predominated by C16: 0, C18: 1!7c and C16: 1!7c. The major polar lipid was phosphatidylethanolamine. Strains DM1 and DM2T had DNA G+C contents of 52.0 and 51.8 mol%, respectively, as determined by HPLC analysis. Phylogenetic analyses based on 16S rRNA gene sequences clearly indicated that the two isolates formed a distinct genus-level lineage in the family Aeromonadaceae of the class Gammaproteobacteria and was an outgroup with respect to a stable supragenic clade comprising species of the genera Oceanimonas, Oceanisphaera and Zobellella. The phylogenetic data and those from chemotaxonomic, physiological and morphological characterizations support the establishment of a novel species and genus inside the family Aeromonadaceae, for which the name Dongshaea marina gen. nov., sp. nov. is proposed. The type strain is DM2T (=BCRC 81069T =JCM 32096T). ? 2019, Microbiology Society. All rights reserved.
|Appears in Collections:||海洋研究所|
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