https://scholars.lib.ntu.edu.tw/handle/123456789/572720
標題: | Differences in anti-inflammatory properties of water soluble and insoluble bioactive polysaccharides in lipopolysaccharide-stimulated RAW264.7 macrophages | 作者: | Su C.-H Tseng Y.-T Lo K.-Y Lai M.-N Ng L.-T. KAI-YIN LO |
關鍵字: | beta glucan; dectin 1; gf 100; gf 121; gf 70; GFP; immunoglobulin enhancer binding protein; interleukin 6; laminaran; nitric oxide; polysaccharide; prostaglandin E2; transcription factor RelA; tumor necrosis factor; unclassified drug; zymosan; antiinflammatory activity; Article; cell viability; concentration response; controlled study; cytokine production; cytokine release; down regulation; drug effect; drug potency; drug protein binding; enzyme activation; gene expression regulation; gene translocation; human; human cell; IC50; macrophage; molecular weight; priority journal; protein expression; RAW 264.7 cell line | 公開日期: | 2020 | 卷: | 37 | 期: | 5 | 起(迄)頁: | 565-576 | 來源出版物: | Glycoconjugate Journal | 摘要: | β-Linked polysaccharides including β-glucans are well known to be important functional ingredients, and are known to possess immunomodulatory and anti-tumor activities. This study aimed to investigate the anti-inflammatory properties and participating receptor of water soluble and insoluble bioactive polysaccharides from Grifola frondosa (GFP, non-digestible water soluble polysaccharides), Laminaria digitata (laminarin, a water soluble β-glucan) and Saccharomyces cerevisiae (zymosan, a water insoluble β-glucan) in lipopolysaccharide (LPS)-stimulated parental and Dectin-1 highly expressing RAW264.7 macrophages. Results showed that GFP and laminarin significantly inhibited nitric oxide and prostaglandin E2 production, but only the GFP with high molecular weight exhibited strong inhibition on pro-inflammatory cytokine (TNF-α and IL-6) secretion in a concentration-dependent manner. The activation of NF-κB was also significantly down-regulated by GFP treatment as compared with cells treated with LPS alone. Although GFP and laminarin were able to bind to β-glucan receptor Dectin-1, there was no relationship between the inhibitory potency and the content of β-glucans in GFP, and these inhibitory effects were not affected by the expression level of Dectin-1 in macrophage cells. In contrast, zymosan significantly intensified LPS-induced inflammatory responses through Dectin-1. In conclusion, these results suggest that the inhibitory effects of water soluble polysaccharides on LPS-induced pro-inflammatory mediator production in murine macrophages may not involve β-glucan receptor Dectin-1. ? 2020, Springer Science+Business Media, LLC, part of Springer Nature. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087909679&doi=10.1007%2fs10719-020-09934-y&partnerID=40&md5=4c6d6c541e58018f059d8035dbbd4eee https://scholars.lib.ntu.edu.tw/handle/123456789/572720 |
ISSN: | 2820080 | DOI: | 10.1007/s10719-020-09934-y |
顯示於: | 農業化學系 |
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