https://scholars.lib.ntu.edu.tw/handle/123456789/572811
標題: | Research progress on CRISPR/Cas9-mediated genome editing technique in plants | 作者: | Shi H Lin Y Lai Z YI-YIN DO Huang P. |
公開日期: | 2018 | 卷: | 24 | 期: | 3 | 起(迄)頁: | 640-650 | 來源出版物: | Chinese Journal of Applied and Environmental Biology | 摘要: | Many physical, chemical, and biotic stresses always threaten plants during their growth and development. Using the genome editing technique is a good strategy to improve plant characteristics. CRISPR/Cas (clustered regulatory interspaced short palindromic repeats / CRISPR-associated protein), the adaptive and heritable immune system of prokaryotes, is a genome editing technique newly developed during recent years. At present, the type II CRISPR / Cas9 system is most widely used. Many plants, such as Arabidopsis thaliana, tobacco, soybean, tomato, potato, rice, wheat, maize, sorghum, petunia, banana, sweet orange, apple, poplar, and Marchantia polymorpha, have been edited successfully for stress tolerance, delay of fruit ripening, herbicide resistance, and disease resistance, etc. This system is achieved by the formation of nuclease Cas9 and crRNA: tracrRNA (CRISPR-derived RNA:trans-activating RNA) complex. Further studies about the effect of the promoters of Cas9 and gRNA and the number of gRNA targets on gene editing efficiency have been discussed. ? 2018 Science Press. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049851959&doi=10.19675%2fj.cnki.1006-687x.2017.07019&partnerID=40&md5=6ab10466518f9f111d195db45600b6a2 https://scholars.lib.ntu.edu.tw/handle/123456789/572811 |
ISSN: | 1006687X | DOI: | 10.19675/j.cnki.1006-687x.2017.07019 |
顯示於: | 園藝暨景觀學系 |
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