|Title:||Novel low-voltage electro-ejaculation approach for sperm collection from zoo captive Lanyu miniature pigs (Sus barbatus sumatranus)||Authors:||Chen Y.-H
|Keywords:||acrosome; article; cell viability; cryopreservation; ejaculation; human cell; male; minipig; mitochondrion; nonhuman; physical stress; pig; seminal plasma; spermatozoon density||Issue Date:||2020||Journal Volume:||10||Journal Issue:||10||Start page/Pages:||1-13||Source:||Animals||Abstract:||
Semen collection can be achieved via hand penile massage or rectal stimulation using electro-ejaculation methods. Traditional electro-ejaculation procedure applied relatively high voltage of 3–15 volts with a maximum current of 900 mA. However, these manipulations often result in great stress and discomforts in animals. In this study, we showed low-voltage electro-ejaculation procedure using 2–3 volts with a maximum current of 500 mA can efficiently stimulated ejaculations in zoo captive lanyu miniature pigs with a high success rate of 81.3% (13/16). Besides normal semen properties (semen volume, pH, sperm concentration), we demonstrated that low-voltage electro-ejaculation caused less stress in the animals, and sperm cells obtained via low-voltage electro-ejaculation exhibit low abnormality (10.3%), high viability (84.3%), motility (75.7%), progressive motility (63.7%), and acrosome integrity (88%). However, cryopreservation protocol used in the current study requires further optimization, as sperm mitochondrial function was partially compromised during freezing procedures. Taken together, we demonstrated in this study that a low-voltage electro-ejaculation approach can be used to obtain quality sperm cells from zoo captive lanyu miniature pig with less physical stress during electro-ejaculation procedure. ? 2020 by the authors. Licensee MDPI, Basel, Switzerland.
|Appears in Collections:||臨床動物醫學研究所|
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