https://scholars.lib.ntu.edu.tw/handle/123456789/575804
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chauhan A | en_US |
dc.contributor.author | Dhenadhayalan N | en_US |
dc.contributor.author | KING-CHUEN LIN | en_US |
dc.date.accessioned | 2021-08-03T01:43:16Z | - |
dc.date.available | 2021-08-03T01:43:16Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 9254005 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85098118990&doi=10.1016%2fj.snb.2020.129284&partnerID=40&md5=423434f1f9d9213caa4096116c6ba682 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/575804 | - |
dc.description.abstract | Evanescent wave cavity ring-down spectroscopy (EW-CRDS) is implemented as a sensitive probe for the detection of prostate-specific antigen (PSA) with a sub-femtomolar limit of detection (LOD). PSA, a glycoprotein, is an essential biomarker for scrutinizing prostate cancer. Gold nanoparticles (Au NPs) were synthesized to serve as a sensitive reporter which are strongly bound to the thiol-modified target DNA. While Au NPs/target DNA flowed via a microfluidic channel, the target DNA may hybridize with aptamer DNA immobilized on the silica surface. Thus, the adsorption kinetics of hybridization between the Au NPs/target and aptamer was measured with interfacial optical absorbance using Langmuir fit of adsorption equilibrium. The LOD of Au NPs/PSA sensor reaches 0.54 fM signifying tremendous detecting proficiency of EW-CRDS. The evanescent wave is highly sensitive for small changes in absorption of material leading to a significant variation of the ring-down time constant. Moreover, Au NPs possess inherent excellent surface plasmonic properties that made them a promising probe material for the interfacial sensing process. To assess the significance of probe material, 5-carboxytetramethylrhodamine was substituted as a reporter to determine sensing kinetics, showing LOD of 99.1 fM. EW-CRDS approach could be used as an efficient sensing technique for the examination of malignant diseases and many biological reactions. ? 2020 Elsevier B.V. | - |
dc.relation.ispartof | Sensors and Actuators, B: Chemical | - |
dc.subject | Absorption spectroscopy; Band structure; Diseases; DNA; Electromagnetic wave reflection; Gold nanoparticles; Light measurement; Probes; Silica; Surface plasmons; Synthesis (chemical); Urology; Adsorption equilibria; Adsorption kinetics; Biological reaction; Evanescent-wave cavity ring-down spectroscopy; Microfluidic channel; Plasmonic properties; Prostate specific antigen; Sensing techniques; Antigens | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Absorption spectroscopy; Band structure; Diseases; DNA; Electromagnetic wave reflection; Gold nanoparticles; Light measurement; Probes; Silica; Surface plasmons; Synthesis (chemical); Urology; Adsorption equilibria; Adsorption kinetics; Biological reactio | - |
dc.title | Evanescent wave cavity ring-down spectroscopy based interfacial sensing of prostate-specific antigen | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.snb.2020.129284 | - |
dc.identifier.scopus | 2-s2.0-85098118990 | - |
dc.relation.journalvolume | 330 | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.orcid | 0000-0002-4933-7566 | - |
顯示於: | 化學系 |
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