https://scholars.lib.ntu.edu.tw/handle/123456789/577304
Title: | Fabrication of Stromal Cell-Derived Factor-1 Contained in Gelatin/Hyaluronate Copo006Cymer Mixed with Hydroxyapatite for Use in Traumatic Bone Defects | Authors: | YUN-LIANG CHANG Hsieh, Chia-Ying Yeh, Chao-Yuan CHIH-HAO CHANG FENG-HUEI LIN |
Keywords: | biomaterial; bone defect; bone regeneration; gelatin; hyaluronate; hydroxyapatite; mesenchymal stem cells; tissue engineering | Issue Date: | 14-Jul-2021 | Journal Volume: | 12 | Journal Issue: | 7 | Source: | Micromachines | Abstract: | Bone defects of orthopedic trauma remain a challenge in clinical practice. Regarding bone void fillers, besides the well-known osteoconductivity of most bone substitutes, osteoinductivity has also been gaining attention in recent years. It is known that stromal cell-derived factor-1 (SDF-1) can recruit mesenchymal stem cells (MSCs) in certain circumstances, which may also play an important role in bone regeneration. In this study, we fabricated a gelatin/hyaluronate (Gel/HA) copolymer mixed with hydroxyapatite (HAP) and SDF-1 to try and enhance bone regeneration in a bone defect model. After material characterization, these Gel/HA-HAP and Gel/HA-HAP-SDF-1 composites were tested for their biocompatibility and ability to recruit MSCs in vitro. A femoral condyle bone defect model of rats was used for in vivo studies. For the assessment of bone healing, micro-CT analysis, second harmonic generation (SHG) imaging, and histology studies were performed. As a result, the Gel/HA-HAP composites showed no systemic toxicity to rats. Gel/HA-HAP composite groups both showed better bone generation compared with the control group in an animal study, and the composite with the SDF-1 group even showed a trend of faster bone growth compared with the composite without SDF-1 group. In conclusion, in the management of traumatic bone defects, Gel/HA-HAP-SDF-1 composites can be a feasible material for use as bone void fillers. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/577304 | ISSN: | 2072-666X | DOI: | 10.3390/mi12070822 |
Appears in Collections: | 醫學系 |
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