https://scholars.lib.ntu.edu.tw/handle/123456789/577318
標題: | The Development of Gelatin/Hyaluronate Copolymer Mixed with Calcium Sulfate, Hydroxyapatite, and Stromal-Cell-Derived Factor-1 for Bone Regeneration Enhancement | 作者: | YUN-LIANG CHANG Hsieh, Chia-Ying Yeh, Chao-Yuan FENG-HUEI LIN |
關鍵字: | SDF-1; biomaterial; bone defect; bone regeneration; calcium sulfate; gelatin; hyaluronate; hydroxyapatite; mesenchymal stem cells | 公開日期: | 5-九月-2019 | 卷: | 11 | 期: | 9 | 來源出版物: | Polymers | 摘要: | In clinical practice, bone defects still remain a challenge. In recent years, apart from the osteoconductivity that most bone void fillers already provide, osteoinductivity has also been emphasized to promote bone healing. Stromal-cell-derived factor-1 (SDF-1) has been shown to have the ability to recruit mesenchymal stem cells (MSCs), which play an important role in the bone regeneration process. In this study, we developed a gelatin-hyaluronate (Gel-HA) copolymer mixed with calcium sulfate (CS), hydroxyapatite (HAP), and SDF-1 in order to enhance bone regeneration in a bone defect model. The composites were tested in vitro for biocompatibility and their ability to recruit MSCs after material characterization. For the in vivo test, a rat femoral condyle bone defect model was used. Micro computed tomography (Micro-CT), two-photon excitation microscopy, and histology analysis were performed to assess bone regeneration. As expected, enhanced bone regeneration was well observed in the group filled with Gel-HA/CS/HAP/SDF-1 composites compared with the control group in our animal model. Furthermore, detailed blood analysis of rats showed no obvious systemic toxicity or side effects after material implantation. In conclusion, the Gel-HA/CS/HAP/SDF-1 composite may be a safe and applicable material to enhance bone regeneration in bone defects. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/577318 | ISSN: | 2073-4360 | DOI: | 10.3390/polym11091454 |
顯示於: | 醫學系 |
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