https://scholars.lib.ntu.edu.tw/handle/123456789/577426
標題: | Label-free platform on pH-responsive chitosan: Adhesive heterogeneity for cancer stem-like cell isolation from A549 cells via integrin β4 | 作者: | Yen, Chia-Hsiang Li, Sheng-Tien NAI-CHEN CHENG Ji, You-Ren JYH-HORNG WANG TAI-HORNG YOUNG |
關鍵字: | Cancer stem-like cell (CSLC); Cell adhesive strength; Integrin β4; Label-free isolation; pH-responsive chitosan | 公開日期: | 1-七月-2020 | 卷: | 239 | 來源出版物: | Carbohydrate polymers | 摘要: | Great efforts have been paid to develop methodologies for cancer stem-like cell (CSLC) isolation in anti-cancer research. The major obstacle lies in the lack of generic biomarkers for different cancer types and the requirement of complicated immuno-labeling procedures. The purpose of this study is to establish a label-free platform for CSLC isolation using pH-responsive chitosan. Based on the adhesive heterogeneity, 15.7 ± 1.9 % of human non-small cell lung cancer (NSCLC) cell line A549 detached from the chitosan substrate following medium pH elevation from 6.99 to 7.65 within 1 h. As a result, this subpopulation of cells with low adhesiveness exhibited superior CSLC hallmarks, including self-renewal, invasive and metastatic potential, therapeutic-resistance, colony formation in vitro, as well as nude mice xenograft in vivo for tumorigenesis, in comparison with their high-adhesive counterpart. Furthermore, integrin β4 is decisive in controlling CSLC detachment of NSCLC. Conclusively, this pH-dependent isolation provides new insights into biomaterial-based CSLC isolation. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/577426 | ISSN: | 01448617 | DOI: | 10.1016/j.carbpol.2020.116168 | SDG/關鍵字: | Cell culture; Cells; Chitosan; Diseases; Drug products; Mammals; Cancer stem-like cell (CSLC); Cell-adhesives; Integrins; Label free; PH-responsive; Adhesives; beta4 integrin; chitosan; A-549 cell line; animal; cancer stem cell; cell adhesion; cell separation; chemistry; experimental neoplasm; female; human; lung tumor; metabolism; mouse; non small cell lung cancer; nude mouse; particle size; pathology; pH; surface property; tumor cell culture; A549 Cells; Animals; Carcinoma, Non-Small-Cell Lung; Cell Adhesion; Cell Separation; Chitosan; Female; Humans; Hydrogen-Ion Concentration; Integrin beta4; Lung Neoplasms; Mice; Mice, Nude; Neoplasms, Experimental; Neoplastic Stem Cells; Particle Size; Surface Properties; Tumor Cells, Cultured |
顯示於: | 醫學系 |
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