https://scholars.lib.ntu.edu.tw/handle/123456789/578364
標題: | Commensal bacterial endocytosis in epithelial cells is dependent on myosin light chain kinase-activated brush border fanning by interferon-γ | 作者: | Wu L.-L. Peng W.-H. WEI-TING KUO Huang C.-Y. YEN-HSUAN NI KUO-SHYAN LU Turner J.R. LINDA CHIA-HUI YU |
公開日期: | 2014 | 出版社: | Elsevier Inc. | 卷: | 184 | 期: | 8 | 起(迄)頁: | 2260-2274 | 來源出版物: | American Journal of Pathology | 摘要: | Abnormal bacterial adherence and internalization in enterocytes have been documented in Crohn disease, celiac disease, surgical stress, and intestinal obstruction and are associated with low-level interferon (IFN)-γ production. How commensals gain access to epithelial soma through densely packed microvilli rooted on the terminal web (TW) remains unclear. We investigated molecular and ultrastructural mechanisms of bacterial endocytosis, focusing on regulatory roles of IFN-γ and myosin light chain kinase (MLCK) in TW myosin phosphorylation and brush border fanning. Mouse intestines were sham operated on or obstructed for 6 hours by loop ligation with intraluminally administered ML-7 (a MLCK inhibitor) or Y27632 (a Rho-associated kinase inhibitor). After intestinal obstruction, epithelial endocytosis and extraintestinal translocation of bacteria were observed in the absence of tight junctional damage. Enhanced TW myosin light chain phosphorylation, arc formation, and brush border fanning coincided with intermicrovillous bacterial penetration, which were inhibited by ML-7 and neutralizing anti-IFN-γ but not Y27632. The phenomena were not seen in mice genetically deficient for long MLCK-210 or IFN-γ. Stimulation of human Caco-2BBe cells with IFN-γ caused MLCK-dependent TW arc formation and brush border fanning, which preceded caveolin-mediated bacterial internalization through cholesterol-rich lipid rafts. In conclusion, epithelial MLCK-activated brush border fanning by IFN-γ promotes adherence and internalization of normally noninvasive enteric bacteria. Transcytotic commensal penetration may contribute to initiation or relapse of chronic inflammation. ? 2014 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84904599137&doi=10.1016%2fj.ajpath.2014.05.003&partnerID=40&md5=189e82f36bd8bd90b375926f141b0741 https://scholars.lib.ntu.edu.tw/handle/123456789/578364 |
ISSN: | 0002-9440 | DOI: | 10.1016/j.ajpath.2014.05.003 | SDG/關鍵字: | DNA 16S; fodrin; gamma interferon; myosin; myosin light chain kinase; protein ZO1; gamma interferon; myosin light chain kinase; animal experiment; animal tissue; article; bacterial overgrowth; bacterial translocation; bacterium adherence; brush border; controlled study; endocytosis; enzyme linked immunosorbent assay; fluorescence in situ hybridization; immunoelectron microscopy; internalization; intestine epithelium cell; intestine loop; intestine obstruction; male; mouse; nonhuman; priority journal; protein phosphorylation; scanning electron microscopy; small intestine; transmission electron microscopy; animal; Bagg albino mouse; C57BL mouse; cell line; confocal microscopy; disease model; electron microscopy; endocytosis; Escherichia coli; fluorescent antibody technique; human; intestine mucosa; intestine obstruction; metabolism; microbiology; microvillus; physiology; symbiosis; ultrastructure; Western blotting; Animals; Blotting, Western; Cell Line; Disease Models, Animal; Endocytosis; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Fluorescent Antibody Technique; Humans; In Situ Hybridization, Fluorescence; Interferon-gamma; Intestinal Mucosa; Intestinal Obstruction; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Microscopy, Confocal; Microscopy, Electron; Microvilli; Myosin-Light-Chain Kinase; Symbiosis |
顯示於: | 口腔生物科學研究所 |
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