https://scholars.lib.ntu.edu.tw/handle/123456789/583088
Title: | Differed IL-1 Beta Response between Active TB and LTBI Cases by Ex Vivo Stimulation of Human Monocyte-Derived Macrophage with TB-Specific Antigen | Authors: | MENG-RUI LEE LIH-YU CHANG Chang C.-H. BO-SHIUN YAN JANN-YUAN WANG Lin W.-H. |
Issue Date: | 2019 | Publisher: | Hindawi Limited | Journal Volume: | 2019 | Start page/Pages: | 7869576 | Source: | Disease Markers | Abstract: | Background. The difference of macrophage-specific interleukin-1 beta (IL-1b) response between latent tuberculosis infection (LTBI) and active tuberculosis (TB) remains less studied. Method. We performed this prospective study and recruited active TB patients, contacts with LTBI, and uninfected contacts. The gene and protein expression of human monocyte-derived macrophage (hMDM) after ex vivo stimulation by early secretory antigenic target-6KD (ESAT-6) and tuberculin purified protein derivatives (PPD) was studied by real-Time PCR and flow cytometry. The effect of caspase-1 inhibitor was also studied. Result. The IL-1b gene expression after 6 hr ESAT-6 1 μg/ml stimulation was different among active TB patients (n=12), LTBI cases (n=12), and uninfected contacts (n=23) (log fold change: 0.98±1.26 vs. 2.20±0.96 vs. 2.20±0.96, P=0.013). The IL-1b gene expression at 24 hours was higher than that at 6 hours in LTBI cases (n=4) and uninfected contacts (n=6). After 24 hr ESAT-6 1 μg/ml stimulation, the percentage of IL-1b-expressed hMDM was borderline lower in the active TB patients (n=9) than in the LTBI cases (n=10) (14.0±11.2% vs. 31.6±22.5%, P=0.065). Compared with ESAT-6 1 μg/ml stimulation but without the addition of caspase-1 inhibitor (CasI) (55.6±16.3%), the percentage of IL-1b-positive hMDMs decreased after addition of CasI (50 μg/ml CasI: 49.8±18.2%, P=0.078; 100 μg/ml CasI: 46.6±20.8%, P=0.030; 150 μg/ml CasI: 33.7±15.5%, P=0.016). Conclusions. This study revealed that macrophage-specific IL-1b response differed among different stages of Mycobacterium tuberculosis infection. The role of IL-1b and inflammasome in the process of LTBI progressing to active TB warrants further investigation. ? 2019 Meng-Rui Lee et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075035036&doi=10.1155%2f2019%2f7869576&partnerID=40&md5=1367eec6f1fa81f8686f0a67b157c1af https://scholars.lib.ntu.edu.tw/handle/123456789/583088 |
ISSN: | 0278-0240 | DOI: | 10.1155/2019/7869576 | SDG/Keyword: | caspase inhibitor; early secretory antigenic target 6; inflammasome; interleukin 1beta; tuberculin; tumor necrosis factor; bacterial antigen; bacterial protein; biological marker; ESAT-6 protein, Mycobacterium tuberculosis; IL1B protein, human; interleukin 1beta; tumor necrosis factor; adult; aged; antigen specificity; Article; enzyme inhibition; ex vivo study; flow cytometry; gene expression; human; human cell; latent tuberculosis; macrophage; monocyte; prospective study; protein expression; real time polymerase chain reaction; tuberculosis; female; follow up; genetics; immunology; latent tuberculosis; macrophage; male; metabolism; microbiology; middle aged; Mycobacterium tuberculosis; prognosis; tuberculosis; very elderly; young adult; Adult; Aged; Aged, 80 and over; Antigens, Bacterial; Bacterial Proteins; Biomarkers; Female; Follow-Up Studies; Humans; Interleukin-1beta; Latent Tuberculosis; Macrophages; Male; Middle Aged; Mycobacterium tuberculosis; Prognosis; Prospective Studies; Tuberculosis; Tumor Necrosis Factor-alpha; Young Adult |
Appears in Collections: | 醫學系 |
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