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  1. NTU Scholars
  2. 醫學院
  3. 法醫學科所
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/586892
DC FieldValueLanguage
dc.contributor.authorLiu, Kuo-Lanen_US
dc.contributor.authorTsai, Li-Chinen_US
dc.contributor.authorLin, Yu-Chihen_US
dc.contributor.authorChang, Yu-Hsuanen_US
dc.contributor.authorHuang, Nu-Enen_US
dc.contributor.authorWu, Fang-Chinen_US
dc.contributor.authorLin, Chun-Yenen_US
dc.contributor.authorHuang, Kuo-Chengen_US
dc.contributor.authorChen, Chiang-Hoen_US
dc.contributor.authorHsieh, Tung-Hoen_US
dc.contributor.authorYang, Lih-Jingen_US
dc.contributor.authorJAMES CHUN-I LEEen_US
dc.contributor.authorLinacre, Adrianen_US
dc.contributor.authorHsieh, Hsing-Meien_US
dc.date.accessioned2021-11-16T05:17:42Z-
dc.date.available2021-11-16T05:17:42Z-
dc.date.issued2021-10-31-
dc.identifier.issn09379827-
dc.identifier.urihttps://scholars.lib.ntu.edu.tw/handle/123456789/586892-
dc.description.abstractIdentification of semen and spermatozoa is crucial in the forensic investigation of alleged sexual assault cases. In cases of alleged sexual assault where there is a long time gap between the incident and sample collection, or in cases of low sperm count, current methods have limitations of specificity, in the case of presumptive tests for semen, or the problem of recording spermatozoa by microscopy if they are few in number. A 3-plex MSRE-PCR (methylation-sensitive restriction enzyme-PCR) assay using a spermatozoa-specific DNA methylated marker to identify spermatozoa has been reported previously by our laboratory. A key advantage over current methods is the increased sensitivity and specificity. A transition from a research tool to operational use requires blind trial testing and inter-laboratory trials. We report on a collaborative exercise where reagents of the 3-plex MSRE-PCR were sent to six participating laboratories. Each laboratory used their own equipment, consumables, and the presumptive reagents conventionally for body fluid (such as acid phosphatase or PSA), DNA extraction, and quantification in practical casework. The reagents and protocol for the 3-plex MSRE-PCR assay and 9 samples were provided by the organizing laboratory. The participating laboratories were requested to fill in the questionnaire after testing. The reported results from all the six participating laboratories were concordant and the expected correct results for the presence of spermatozoa. These outcomes verified the reproducibility and feasibility of the 3-plex MSRE-PCR assay. The results also indicated that the 3-plex MSRE-PCR assay was readily accessible to forensic laboratories for integrating it into current forensic casework processes.en_US
dc.language.isoenen_US
dc.relation.ispartofInternational journal of legal medicineen_US
dc.subject3-plex MSRE-PCR; Collaborative exercise; Sexual assault; Spermatozoa identificationen_US
dc.titleSpermatozoa identification by the 3-plex MSRE-PCR assay: a collaborative exerciseen_US
dc.typejournal articleen_US
dc.identifier.doi10.1007/s00414-021-02737-2-
dc.identifier.pmid34718845-
dc.identifier.scopus2-s2.0-85118302132-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85118302132-
item.openairetypejournal article-
item.languageiso639-1en-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.grantfulltextnone-
item.cerifentitytypePublications-
crisitem.author.deptForensic Medicine-
crisitem.author.orcid0000-0002-1502-8693-
crisitem.author.parentorgCollege of Medicine-
Appears in Collections:法醫學科所
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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

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