https://scholars.lib.ntu.edu.tw/handle/123456789/589286
標題: | Multilocus sequence typing for analyses of clonality of Candida albicans strains in Taiwan | 作者: | Chen K.-W. YEE-CHUN CHEN Lo H.-J. Odds F.C. Wang T.-H. Lin C.-Y. Li S.-Y. |
公開日期: | 2006 | 卷: | 44 | 期: | 6 | 起(迄)頁: | 2172-2178 | 來源出版物: | Journal of Clinical Microbiology | 摘要: | Multilocus sequence typing (MLST) was used to characterize the genetic profiles of 51 Candida albicans isolates collected from 12 hospitals in Taiwan. Among the 51 isolates, 16 were epidemiologically unrelated, 28 were isolates from 11 critically ill, human immunodeficiency virus (HIV)-negative patients, and 7 were long-term serial isolates from 3 HIV-positive patients. Internal regions of seven housekeeping genes were sequenced. A total of 83 polymorphic nucleotide sites were identified. Ten to 20 different genotypes were observed at the different loci, resulting, when combined, in 45 unique genotype combinations or diploid sequence types (DSTs). Thirty (36.1%) of the 83 individual changes were synonymous and 53 (63.9%) were nonsynonymous. Due to the diploid nature of C. albicans, MLST was more discriminatory than the pulsed-field gel electrophoresis-BssHII-restricted fragment method in discriminating epidemiologically related strains. MLST is able to trace the microevolution over time of C. albicans isolates in the same patient. All but one of the DSTs of our Taiwanese strain collections were novel to the internet C. albicans DST database (http://testl.mlst.net/). The DSTs of C. albicans in Taiwan were analyzed together with those of the reference strains and of the strains from the United Kingdom and United States by unweighted-pair group method using average linkages and minimum spanning tree. Our result showed that the DNA type of each isolate was patient specific and associated with ABC type and decade of isolation but not associated with mating type, anatomical source of isolation, hospital origin, or fluconazole resistance patterns. Copyright ? 2006, American Society for Microbiology. All Rights Reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33745160739&doi=10.1128%2fJCM.00320-06&partnerID=40&md5=b32e8dc6974f41f678aa5bb8744f150c https://scholars.lib.ntu.edu.tw/handle/123456789/589286 |
ISSN: | 0095-1137 | DOI: | 10.1128/JCM.00320-06 | SDG/關鍵字: | fluconazole; antibiotic resistance; article; Candida albicans; clonal variation; critical illness; DNA polymorphism; fungal strain; fungus isolation; genotype; hospital infection; housekeeping gene; Human immunodeficiency virus infected patient; mating type; multilocus sequence typing; nonhuman; phylogenetic tree; pleura effusion; priority journal; pulsed field gel electrophoresis; sequence analysis; sputum culture; Taiwan; throat culture; United Kingdom; United States; urine culture; wound fluid; AIDS-Related Opportunistic Infections; Candida albicans; Candidiasis; Electrophoresis, Gel, Pulsed-Field; Fungal Proteins; HIV Seropositivity; Humans; Mycological Typing Techniques; Restriction Mapping; Sequence Analysis, DNA; Species Specificity; Taiwan; Candida albicans; Human immunodeficiency virus |
顯示於: | 醫學系 |
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