https://scholars.lib.ntu.edu.tw/handle/123456789/592676
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Huang Y.-T. | en_US |
dc.contributor.author | Liu M.-Y. | en_US |
dc.contributor.author | CHING-HWA TSAI | en_US |
dc.contributor.author | TE-HUEI YEH | en_US |
dc.date.accessioned | 2022-01-19T09:29:55Z | - |
dc.date.available | 2022-01-19T09:29:55Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1043-3074 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-77954412016&doi=10.1002%2fhed.21270&partnerID=40&md5=943b5b118c353221e6622cc85dfe9d6d | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/592676 | - |
dc.description.abstract | Background. Nasopharyngeal carcinoma (NPC) is associated with Epstein-Barr virus (EBV) infection. We previously found that interleukin (IL)-1α and IL-1β significantly increased in NPC tissues. This study investigated what EBVencoded proteins were involved in such IL-1 production. Methods and Results. IL-1α and IL-1β messenger ribonucleic acids (mRNAs) were detected in the EBV latent membrane protein 1 (LMP1) transfectant (LMP135) only by reverse transcriptase-polymerase chain reaction (RT-PCR). LMP1-mediated IL-1α and IL-1β production could be enhanced by tumor necrosis factor alpha (TNF-α), determined by enzymelinked immunosorbent assay (ELISA). Moreover, IL-1α and IL-1β mRNAs and proteins were increased in a dose-dependent manner in epithelial cells transiently transfected by an LMP1 plasmid. Besides, immortalized human epidermal keratinocyte (RHEK-1) epithelial cells could be enhanced to proliferate by IL-1α and IL-1β determined by water-soluble tetrazolium salt (WST-1) assay. Conclusions. EBV LMP1 is capable of upregulating IL-1α and IL-1β secretions from epithelial cells and positively modulated by TNF-a. This may consequently contribute to tumor growth in patients with NPC. ? 2009 Wiley Periodicals, Inc. | - |
dc.relation.ispartof | Head and Neck | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | interleukin 1alpha; interleukin 1beta; latent membrane protein 1; messenger RNA; tumor necrosis factor alpha; article; cancer cell; cell growth; cell proliferation; controlled study; cytokine production; dose response; enzyme linked immunosorbent assay; epithelium cell; Epstein Barr virus; human; human cell; keratinocyte; nasopharynx carcinoma; nonhuman; priority journal; real time polymerase chain reaction; reverse transcription polymerase chain reaction; upregulation; Carcinoma; Cell Culture Techniques; Cell Line; Cell Proliferation; Epithelial Cells; Herpesvirus 4, Human; Humans; Interleukin-1; Nasopharyngeal Neoplasms; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Viral Matrix Proteins | - |
dc.title | Upregulation of interleukin-1 by Epstein-Barr virus latent membrane protein 1 and its possible role in nasopharyngeal carcinoma cell growth | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/hed.21270 | - |
dc.identifier.pmid | 19827118 | - |
dc.identifier.scopus | 2-s2.0-77954412016 | - |
dc.relation.pages | 869-876 | - |
dc.relation.journalvolume | 32 | - |
dc.relation.journalissue | 7 | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Microbiology | - |
crisitem.author.dept | Otolaryngology | - |
crisitem.author.dept | Otolaryngology-NTUH | - |
crisitem.author.orcid | 0000-0003-3818-2818 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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