|Title:||Canine transmissible venereal tumour established in immunodeficient mice reprograms the gene expression profiles associated with a favourable tumour microenvironment to enable cancer malignancy||Authors:||Ke, Chiao Hsu
Lin, Yu Ling
Huang, Hsiao Hsuan
Chiang, Hsin Chien
|Keywords:||Canine tumour model | cDNA microarray | Immunoediting | Infectious cancer | Tumour biomarkers;Canine tumour model; cDNA microarray; Immunoediting; Infectious cancer; Tumour biomarkers||Issue Date:||1-Dec-2022||Journal Volume:||18||Journal Issue:||1||Source:||BMC Veterinary Research||Abstract:||
Background: Canine transmissible venereal tumours (CTVTs) can cross the major histocompatibility complex barrier to spread among dogs. In addition to the transmissibility within canids, CTVTs are also known as a suitable model for investigating the tumour–host immunity interaction because dogs live with humans and experience the same environmental risk factors for tumourigenesis. Moreover, outbred dogs are more appropriate than inbred mice models for simulating the diversity of human cancer development. This study built a new model of CTVTs, known as MCTVTs, to further probe the shaping effects of immune stress on tumour development. For xenotransplantation, CTVTs were first injected and developed in immunodeficient mice (NOD.CB17-Prkdcscid/NcrCrl), defined as XCTVTs. The XCTVTs harvested from NOD/SCID mice were then inoculated and grown in beagles and named mouse xenotransplantation of CTVTs (MCTVTs). Results: After the inoculation of CTVTs and MCTVTs into immune-competent beagle dogs separately, MCTVTs grew faster and metastasized more frequently than CTVTs did. Gene expression profiles in CTVTs and MCTVTs were analysed by cDNA microarray to reveal that MCTVTs expressed many tumour-promoting genes involved in chronic inflammation, chemotaxis, extracellular space modification, NF-kappa B pathways, and focal adhesion. Furthermore, several well-known tumour-associated biomarkers which could predict tumour progression were overexpressed in MCTVTs. Conclusions: This study demonstrated that defective host immunity can result in gene instability and enable transcriptome reprogramming within tumour cells. Fast tumour growth in beagle dogs and overexpression of tumour-associated biomarkers were found in a CTVT strain previously established in immunodeficient mice. In addition, dysregulated interaction of chronic inflammation, chemotaxis, and extracellular space modification were revealed to imply the possibly exacerbating mechanisms in the microenvironments of these tumours. In summary, this study offers a potential method to facilitate tumour progression and provide a niche for discovering tumour-associated biomarkers in cancer research.
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/592906||DOI:||10.1186/s12917-021-03093-4||metadata.dc.subject.other:||apolipoprotein C1; ELAV like protein 2; interstitial collagenase; kynurenine 3 monooxygenase; tumor marker; animal cell; animal experiment; animal model; animal tissue; Article; beagle; bioinformatics; cancer model; cancer research; Canidae; canine transm
|Appears in Collections:||法律學系|
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