https://scholars.lib.ntu.edu.tw/handle/123456789/593855
標題: | Laminin-Alginate Beads as Preadipocyte Carriers to Enhance Adipogenesis In Vitro and In Vivo | 作者: | Hsueh, Yu-Sheng Chen, Yo-Shen HAO-CHIH TAI Mestak, Ondrej SUNG-CHUAN CHAO Chen, Yen-Yu Shih, Ying Lin, Jung-Feng MING-JIUM SHIEH FENG-HUEI LIN |
關鍵字: | laminin-alginate bead; fat survival; adipogenesis; preadipocytes; cell-assisted lipotransfer; CELL-ASSISTED LIPOTRANSFER; TISSUE ENGINEERING APPLICATIONS; HYDROGELS; RGD; EFFICACY; AUGMENTATION; BIOMATERIALS; ADIPOCYTES; ADHESION; SAFETY | 公開日期: | 三月-2017 | 出版社: | MARY ANN LIEBERT, INC | 卷: | 23 | 期: | 5-6 | 起(迄)頁: | 185 | 來源出版物: | Tissue Engineering Part A | 摘要: | The use of autologous fat grafting in breast reconstruction still requires optimization. Fat survival and calcification are the main issues that affect the outcomes of the procedure. In this study, a cell-based therapy utilizing laminin-alginate beads (LABs) as carriers was proposed to promote cell survival and adipogenesis by providing short-term physical support and facilitate nutrient diffusion of the implants. Laminin-modified alginate beads were fabricated by immobilizing laminin onto ring-opened alginate, used to encapsulate 3T3-L1 preadipocytes, and evaluated in vitro and in vivo. LABs as preadipocyte carriers showed better biocompatibility and stability than unmodified alginate beads. Preadipocytes in LABs had higher survival rate and enhanced adipogenesis than those in unmodified alginate beads. In vivo studies showed that LABs gradually degraded and the sites were replaced by newly formed fat tissues, and new blood vessels were also observed. 7T-MRI study mimicking clinical fat grafting showed that LABs carrying adipose stem cells improved the results of conventional fat grafts. Therefore, we believe that LABs represent promising cell carriers and can be potentially used for the reconstruction of breasts or other soft tissues in the future. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/593855 | ISSN: | 1937-3341 1937-335X |
DOI: | 10.1089/ten.tea.2016.0290 | SDG/關鍵字: | Biocompatibility; Biomineralization; Blood vessels; Cells; Cytology; Histology; Laboratories; Stem cells; Tissue; 3t3-l1 preadipocytes; Adipogenesis; Adipose stem cells; Alginate beads; Breast reconstruction; Cell based therapies; Nutrient diffusion; Preadipocytes; Alginate; alginic acid; laminin; nitrogen; periodate sodium; urea; alginic acid; glucuronic acid; hexuronic acid; laminin; adipogenesis; animal cell; Article; biocompatibility; cell encapsulation; cell structure; cell survival; cell therapy; comparative study; controlled study; cross linking; fat necrosis; female; histology; immobilization; in vitro study; in vivo study; infrared spectroscopy; nonhuman; nuclear magnetic resonance imaging; oxidation; priority journal; proadipocyte; rat; scanning electron microscopy; survival rate; urea nitrogen blood level; 3T3-L1 cell line; adipocyte; animal; chemistry; immobilized cell; metabolism; mouse; nonobese diabetic mouse; SCID mouse; Sprague Dawley rat; transplantation; 3T3-L1 Cells; Adipocytes; Adipogenesis; Alginates; Animals; Cells, Immobilized; Female; Glucuronic Acid; Hexuronic Acids; Laminin; Mice; Mice, Inbred NOD; Mice, SCID; Rats, Sprague-Dawley |
顯示於: | 醫學系 |
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