https://scholars.lib.ntu.edu.tw/handle/123456789/594005
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Luo, Elizabeth Wei-Chia | en_US |
dc.contributor.author | MENG-LIN LIAO | en_US |
dc.contributor.author | CHUNG-LIANG CHIEN | en_US |
dc.date.accessioned | 2022-02-10T06:17:07Z | - |
dc.date.available | 2022-02-10T06:17:07Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/594005 | - |
dc.description.abstract | Glioblastoma is a malignant brain tumor with poor prognosis that rapidly acquires resistance to available clinical treatments. The herpes simplex virus thymidine kinase/ganciclovir (HSVtk/GCV) system produces the selective elimination of HSVtk-positive cells and is a candidate for preclinical testing against glioblastoma via its ability to regulate proliferation and differentiation. Therefore, in this study, we aimed to establish a plasmid encoding the HSVtk/GCV system driven by a glial fibrillary acidic protein (GFAP) promoter and verify its possibility of neural differentiation of glioblastoma cell line under the GCV challenge. Four stable clones-N2A-pCMV-HSVtk, N2A-pGFAP-HSVtk, U251-pCMV-HSVtk, and U251-pGFAP-HSVtk-were established from neuronal N2A and glioblastoma U251 cell lines. In vitro GCV sensitivity was assessed by MTT assay for monitoring time- and dosage-dependent cytotoxicity. The capability for neural differentiation in stable glioblastoma clones during GCV treatment was assessed by performing immunocytochemistry for nestin, GFAP, and βIII-tubulin. Under GFAP promoter control, the U251 stable clone exhibited GCV sensitivity, while the neuronal N2A clones were nonreactive. During GCV treatment, cells underwent apoptosis on day 3 and dying cells were identified after day 5. Nestin was increasingly expressed in surviving cells, indicating that the population of neural stem-like cells was enriched. Lower levels of GFAP expression were detected in surviving cells. Furthermore, βIII-tubulin-positive neuron-like cells were identified after GCV treatment. This study established pGFAP-HSVtk-P2A-EGFP plasmids that successfully ablated GFAP-positive glioblastoma cells, but left neuronal N2A cells intact. These data suggest that the neural differentiation of glioblastoma cells can be promoted by treatment with the HSVtk/GCV system. | en_US |
dc.language.iso | en | en_US |
dc.publisher | PUBLIC LIBRARY SCIENCE | en_US |
dc.relation.ispartof | PloS one | en_US |
dc.subject | CANCER STEM-CELLS; ASTROCYTE-SPECIFIC EXPRESSION; SUICIDE GENE-THERAPY; IDENTIFICATION; CYTOTOXICITY; HSVTK; BRAIN | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | Neural differentiation of glioblastoma cell lines via a herpes simplex virus thymidine kinase/ganciclovir system driven by a glial fibrillary acidic protein promoter | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1371/journal.pone.0253008 | - |
dc.identifier.pmid | 34370752 | - |
dc.identifier.scopus | 2-s2.0-85112273562 | - |
dc.identifier.isi | WOS:000685266800049 | - |
dc.identifier.url | https://scholars.lib.ntu.edu.tw/handle/123456789/582717 | - |
dc.relation.journalvolume | 16 | en_US |
dc.relation.journalissue | 8 | en_US |
item.openairetype | journal article | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
crisitem.author.dept | Anatomy and Cell Biology | - |
crisitem.author.dept | Anatomy and Cell Biology | - |
crisitem.author.dept | Medical Genomics and Proteomics | - |
crisitem.author.orcid | 0000-0002-2293-319X | - |
crisitem.author.orcid | 0000-0001-9806-486X | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 解剖學暨細胞生物學科所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。