|Title:||MicroRNA-17/20a functions to inhibit cell migration and can be used a prognostic marker in oral squamous cell carcinoma||Authors:||Chang C.-C.
|Issue Date:||2013||Publisher:||Elsevier Ltd||Journal Volume:||49||Journal Issue:||9||Start page/Pages:||923-931||Source:||Oral Oncology||Abstract:||
Objectives Oral squamous cell carcinoma (OSCC) accounts for >90% oral cancer which is a leading cause of cancer death worldwide. Early diagnosis may well offer an opportunity to increase survival to this neoplasm. Micro(mi)RNA-interfered cancer progression is crucial, yet its migration machinery of OSCC is still unknown. To access whether the possible miRNA prognostic markers and underlying mechanisms, we developed a highly migratory TW2.6 MS-10 cells from TW2.6 cells to investigate the issue. Materials and methods miRNA profiling was performed on TW2.6 and TW2.6 MS-10. Target miRNA was correlated to pathological status in OSCC patients by real-time RT-PCR. A downstream effector was identified using a bioinformatics analysis, and a 3′-untranslated region (UTR) reporter assay was used. Results An miRNA cluster, miR-17-92, including miR-17, miR-19b, miR-20a, and miR-92a, was found to be significantly down-regulated in TW2.6 MS-10 compared to TW2.6 cells. Overexpression of this cluster decreased the migratory ability of OSCC cell lines. We further demonstrated that miR-17 and miR-20a are the main miRNAs of miR-17-92 cluster which modulate OSCC migration. Clinically, miR-17/20a showed negative correlation with TNM stage and lymphatic metastasis. Through a bioinformatics screening analysis and 3′UTR reporter assay, we confirmed the integrin (ITG) β8 as a direct target of miR-17/20a, and knockdown of ITGβ8 reduced cell migratory capability of OSCC. Conclusions miR-17/20a acts as a prognostic predictor of OSCC patients' outcome and a tumor migration suppressor miRNA. ? 2013 Elsevier Ltd. All rights reserved.
|ISSN:||1368-8375||DOI:||10.1016/j.oraloncology.2013.03.430||metadata.dc.subject.other:||beta integrin; beta8 integrin; microRNA; microRNA 17; microRNA 19b; microRNA 20a; microrna 92a; unclassified drug; 3' untranslated region; article; cancer cell; cancer prognosis; cancer staging; cell migration; controlled study; down regulation; gene expression; human; human cell; lymph node metastasis; major clinical study; mouth squamous cell carcinoma; priority journal; reverse transcription polymerase chain reaction; 3′ untranslated region; 3′UTR; comparative threshold; CT; ingenuity pathway analysis; integrin β8; IPA; ITGβ8; LAMA3; laminin α3; microRNA; Migration; miRNA; miRNA-17; miRNA-20a; oral squamous cell carcinoma; Oral squamous cell carcinoma; OSCC; receiver-operating characteristics; ROC; Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Movement; Cluster Analysis; Humans; MicroRNAs; Mouth Neoplasms; Prognosis
|Appears in Collections:||醫學系|
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