https://scholars.lib.ntu.edu.tw/handle/123456789/606121
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Sanobar N | en_US |
dc.contributor.author | Lin P.-C | en_US |
dc.contributor.author | Pan Z.-J | en_US |
dc.contributor.author | Fang R.-Y | en_US |
dc.contributor.author | Tjita V | en_US |
dc.contributor.author | Chen F.-F | en_US |
dc.contributor.author | Wang H.-C | en_US |
dc.contributor.author | Tsai H.-L | en_US |
dc.contributor.author | Wu S.-H | en_US |
dc.contributor.author | Shen T.-L | en_US |
dc.contributor.author | Chen Y.-H | en_US |
dc.contributor.author | TANG-LONG SHEN | en_US |
dc.contributor.author | SHIH-SHUN LIN | en_US |
dc.contributor.author | HUANG-LUNG TSAI | en_US |
dc.creator | Sanobar N;Lin P.-C;Pan Z.-J;Fang R.-Y;Tjita V;Chen F.-F;Wang H.-C;Tsai H.-L;Wu S.-H;Shen T.-L;Chen Y.-H;Lin S.-S. | - |
dc.date.accessioned | 2022-04-25T06:13:01Z | - |
dc.date.available | 2022-04-25T06:13:01Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 19994915 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85115410157&doi=10.3390%2fv13091837&partnerID=40&md5=3874dc267afcc15ae995c2f64ba84e0d | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/606121 | - |
dc.description.abstract | In plants, HEN1-facilitated methylation at 3′ end ribose is a critical step of small-RNA (sRNA) biogenesis. A mutant of well-studied Arabidopsis HEN1 (AtHEN1), hen1-1, showed a defective developmental phenotype, indicating the importance of sRNA methylation. Moreover, Marchantia polymorpha has been identified to have a HEN1 ortholog gene (MpHEN1); however, its function remained unfathomed. Our in vivo and in vitro data have shown MpHEN1 activity being comparable with AtHEN1, and their substrate specificity towards duplex microRNA (miRNA) remained consistent. Furthermore, the phylogenetic tree and multiple alignment highlighted the conserved molecular evolution of the HEN1 family in plants. The P1/HC-Pro of the turnip mosaic virus (TuMV) is a known RNA silencing suppressor and inhibits HEN1 methylation of sRNAs. Here, we report that the HC-Pro physically binds with AtHEN1 through FRNK motif, inhibiting HEN1’s methylation activity. Moreover, the in vitro EMSA data indicates GST-HC-Pro of TuMV lacks sRNA duplex-binding ability. Surprisingly, the HC-Pro also inhibits MpHEN1 activity in a dosage-dependent manner, suggesting the possibility of interaction between HC-Pro and MpHEN1 as well. Further investigations on understanding interaction mechanisms of HEN1 and various HC-Pros can advance the knowledge of viral suppressors. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.relation.ispartof | Viruses | - |
dc.subject | HEN1 | - |
dc.subject | HEN1-HC-Pro interaction | - |
dc.subject | Methylation | - |
dc.subject | SRNA | - |
dc.subject | Viral suppressors | - |
dc.subject | Arabidopsis protein | - |
dc.subject | microRNA | - |
dc.subject | recombinant protein | - |
dc.subject | small interfering RNA | - |
dc.subject | cysteine proteinase | - |
dc.subject | HC-Pro protein, potyvirus | - |
dc.subject | HEN1 protein, Arabidopsis | - |
dc.subject | methyltransferase | - |
dc.subject | plant protein | - |
dc.subject | plant RNA | - |
dc.subject | protein binding | - |
dc.subject | viral protein | - |
dc.subject | amino acid sequence | - |
dc.subject | angiosperm | - |
dc.subject | Arabidopsis | - |
dc.subject | Article | - |
dc.subject | binding affinity | - |
dc.subject | biogenesis | - |
dc.subject | chemoluminescence | - |
dc.subject | controlled study | - |
dc.subject | DNA methylation | - |
dc.subject | enzyme specificity | - |
dc.subject | Escherichia coli | - |
dc.subject | female | - |
dc.subject | gel mobility shift assay | - |
dc.subject | gene | - |
dc.subject | gene duplication | - |
dc.subject | gene silencing | - |
dc.subject | immunization | - |
dc.subject | in vitro study | - |
dc.subject | in vivo study | - |
dc.subject | male | - |
dc.subject | molecular evolution | - |
dc.subject | mosaicism | - |
dc.subject | moss | - |
dc.subject | MpHEN1 gene | - |
dc.subject | nonhuman | - |
dc.subject | nucleosome | - |
dc.subject | phenotype | - |
dc.subject | phylogenetic tree | - |
dc.subject | phylogeny | - |
dc.subject | plant growth | - |
dc.subject | plant immunity | - |
dc.subject | polyacrylamide gel electrophoresis | - |
dc.subject | protein degradation | - |
dc.subject | protein expression | - |
dc.subject | protein motif | - |
dc.subject | protein purification | - |
dc.subject | RNA extraction | - |
dc.subject | RNA methylation | - |
dc.subject | sequence alignment | - |
dc.subject | Western blotting | - |
dc.subject | chemistry | - |
dc.subject | genetics | - |
dc.subject | Marchantia | - |
dc.subject | metabolism | - |
dc.subject | methylation | - |
dc.subject | Potyvirus | - |
dc.subject | protein domain | - |
dc.subject | virology | - |
dc.subject | Amino Acid Motifs | - |
dc.subject | Arabidopsis Proteins | - |
dc.subject | Cysteine Endopeptidases | - |
dc.subject | Methyltransferases | - |
dc.subject | MicroRNAs | - |
dc.subject | Phylogeny | - |
dc.subject | Plant Proteins | - |
dc.subject | Protein Binding | - |
dc.subject | Protein Domains | - |
dc.subject | Recombinant Proteins | - |
dc.subject | RNA, Plant | - |
dc.subject | Substrate Specificity | - |
dc.subject | Viral Proteins | - |
dc.title | Investigating the viral suppressor HC-pro inhibiting small rna methylation through functional comparison of HEN1 in angiosperm and bryophyte | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.3390/v13091837 | - |
dc.identifier.pmid | 34578418 | - |
dc.identifier.scopus | 2-s2.0-85115410157 | - |
dc.relation.journalvolume | 13 | - |
dc.relation.journalissue | 9 | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Plant Pathology and Microbiology | - |
crisitem.author.dept | Biotechnology | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Molecular and Cellular Biology | - |
crisitem.author.dept | Life Science | - |
crisitem.author.orcid | 0000-0001-6264-3608 | - |
crisitem.author.orcid | 0000-0002-7295-5004 | - |
crisitem.author.orcid | 0000-0003-3467-0507 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | College of Life Science | - |
顯示於: | 植物病理與微生物學系 |
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