https://scholars.lib.ntu.edu.tw/handle/123456789/606152
Title: | A natural degradant of curcumin, feruloylacetone inhibits cell proliferation via inducing cell cycle arrest and a mitochondrial apoptotic pathway in hct116 colon cancer cells | Authors: | Chou Y.-T Koh Y.-C Nagabhushanam K Ho C.-T MIN-HSIUNG PAN |
Keywords: | Colon cancer;Curcumin;Degradant;Demethoxy-feruloylacetone;Feruloylacetone;antineoplastic agent;antioxidant;CCNB1 protein, human;CDKN1A protein, human;curcumin;cyclin B1;cyclin dependent kinase inhibitor 1A;feruloylacetone;MTOR protein, human;phenol;protein p53;reactive oxygen metabolite;STAT3 protein;STAT3 protein, human;styrene derivative;target of rapamycin kinase;apoptosis;cell cycle checkpoint;cell cycle G2 phase;cell division;cell proliferation;chemistry;colon tumor;drug effect;HCT 116 cell line;human;metabolism;mitochondrial membrane potential;mitochondrion;pathology;phosphorylation;Antineoplastic Agents;Antioxidants;Apoptosis;Cell Cycle Checkpoints;Cell Division;Cell Proliferation;Colonic Neoplasms;Cyclin B1;Cyclin-Dependent Kinase Inhibitor p21;G2 Phase;HCT116 Cells;Humans;Membrane Potential, Mitochondrial;Mitochondria;Phenol;Phosphorylation;Reactive Oxygen Species;STAT3 Transcription Factor;Styrenes;TOR Serine-Threonine Kinases;Tumor Suppressor Protein p53 | Issue Date: | 2021 | Journal Volume: | 26 | Journal Issue: | 16 | Source: | Molecules | Abstract: | Feruloylacetone (FER) is a natural degradant of curcumin after heating, which structurally reserves some functional groups of curcumin. It is not as widely discussed as its original counterpart has been previously; and in this study, its anticancer efficacy is investigated. This study focuses on the suppressive effect of FER on colon cancer, as the efficacious effect of curcumin on this typical cancer type has been well evidenced. In addition, demethoxy-feruloylacetone (DFER) was applied to compare the effect that might be brought on by the structural differences of the methoxy group. It was revealed that both FER and DFER inhibited the proliferation of HCT116 cells, possibly via suppression of the phosphorylated mTOR/STAT3 pathway. Notably, FER could significantly repress both the STAT3 phosphorylation and protein levels. Furthermore, both samples showed capability of arresting HCT116 cells at the G2/M phase via the activation of p53/p21 and the upregulation of cyclin-B. In addition, ROS elevation and changes in mitochondrial membrane potential were revealed, as indicated by p-atm elevation. The apoptotic rate rose to 36.9 and 32.2% after being treated by FER and DFER, respectively. In summary, both compounds exhibited an anticancer effect, and FER showed a greater proapoptotic effect, possibly due to the presence of the methoxy group on the aromatic ring. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85112688542&doi=10.3390%2fmolecules26164884&partnerID=40&md5=2f0dbd77149252e4dffb44a772689431 https://scholars.lib.ntu.edu.tw/handle/123456789/606152 |
ISSN: | 14203049 | DOI: | 10.3390/molecules26164884 |
Appears in Collections: | 食品科技研究所 |
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