https://scholars.lib.ntu.edu.tw/handle/123456789/606390
Title: | Vibrio nitrifigilis sp. nov., a marine nitrogen-fixing bacterium isolated from the lagoon sediment of an islet inside an atoll | Authors: | TZONG-HUEI LEE PEI-LING WANG |
Keywords: | Marine bacteria;Multilocus sequence analysis (MLSA);Nitrogen-fixing bacteria;Vibrio;Vibrio nitrifigilis;Vibrionaceae;carbohydrate;fatty acid;glucose;nitrogen;phosphatidylethanolamine;phosphatidylserine;sodium chloride;bacterial DNA;phospholipid;RNA 16S;Article;bacterial gene;bacterial strain;bacterium isolation;controlled study;DNA sequencing;ftsZ gene;gapA gene;genetic analysis;gyrB gene;housekeeping gene;lagoon;mreB gene;multilocus sequence typing;nonhuman;sediment;topA gene;bacterium identification;DNA base composition;DNA sequence;genetics;phylogeny;Bacterial Typing Techniques;Base Composition;DNA, Bacterial;Fatty Acids;Nitrogen;Phospholipids;Phylogeny;RNA, Ribosomal, 16S;Sequence Analysis, DNA | Issue Date: | 2021 | Publisher: | Springer Science and Business Media Deutschland GmbH | Journal Volume: | 114 | Journal Issue: | 7 | Start page/Pages: | 933-945 | Source: | Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Abstract: | A nitrogen-fixing isolate of facultatively anaerobic, marine bacterium, designated strain NFV-1T, was recovered from the lagoon sediment of Dongsha Island, Taiwan. It was a Gram-negative rod which exhibited motility with monotrichous flagellation in broth cultures. The strain required NaCl for growth and grew optimally at about 25–35?°C, 3% NaCl and pH 7–8. It grew aerobically and could achieve anaerobic growth by fermenting d-glucose or other carbohydrates as substrates. NH4Cl could serve as a sole nitrogen source for growth aerobically and anaerobically, whereas growth with N2 as the sole nitrogen source was observed only under anaerobic conditions. Cellular fatty acids were predominated by C16:1ω7c, C16:0, and C18:1ω7c. The major polar lipids consisted of phosphatidylethanolamine and phosphatidylserine. Strain NFV-1T had a DNA G + C content of 42.5?mol%, as evaluated according to the chromosomal DNA sequencing data. Analyses of sequence similarities and phylogeny based on the 16S rRNA genes, together with the housekeeping genes, gyrB, ftsZ, mreB, topA and gapA, indicated that the strain formed a distinct species-level lineage in the genus Vibrio of the family Vibrionaceae. These phylogenetic data and those from genomic and phenotypic characterisations support the establishment of a novel Vibrio species, for which the name Vibrio nitrifigilis sp. nov. (type strain NFV-1T = BCRC 81211T = JCM 33628T) is proposed. ? 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104995445&doi=10.1007%2fs10482-021-01567-x&partnerID=40&md5=5f0c160f314119ef6ff5ba25ce0d650c https://scholars.lib.ntu.edu.tw/handle/123456789/606390 |
ISSN: | 00036072 | DOI: | 10.1007/s10482-021-01567-x |
Appears in Collections: | 海洋研究所 |
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