https://scholars.lib.ntu.edu.tw/handle/123456789/62530
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 王勝仕 | en |
dc.contributor | 臺灣大學:化學工程學研究所 | zh_TW |
dc.contributor.author | 劉志文 | zh_TW |
dc.contributor.author | Liu, Chih-Wen | en |
dc.creator | 劉志文 | zh_TW |
dc.creator | Liu, Chih-Wen | en |
dc.date | 2007 | en |
dc.date.accessioned | 2007-11-26T04:00:03Z | - |
dc.date.accessioned | 2018-06-28T17:07:15Z | - |
dc.date.available | 2007-11-26T04:00:03Z | - |
dc.date.available | 2018-06-28T17:07:15Z | - |
dc.date.issued | 2007 | - |
dc.identifier | zh-TW | en |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/52281 | - |
dc.description.abstract | 本研究主要利用菌株Rhodococcus erythropolis NTU-1的生物降解及包覆聚集能力來處理碳氫化合物。主要研究目的為探討培養過程中形成結塊包覆正十六烷之原因及其機制。實驗結果發現結塊現象的發生主要和培養基內正十六烷量和細胞量有關(其比值定義為R16)。在本實驗系統中,當R16值小於3.5時,即可能發生結塊現象。形成結塊現象時,培養基不同的R16值會形成不同的結塊構形,可能為土黃色顆粒、片狀、球狀或是乳白色顆粒、球狀。 結塊形成機制為NTU-1細胞在培養過程中以正十六烷作為細胞之間的連結物,ㄧ層一層堆疊,進而形成結塊。菌塊顆粒越大時正十六烷的包覆量越多。當結塊外層大部分為NTU-1細胞時,結塊為土黃色顆粒,而結塊外層大部分為正十六烷時,結塊為乳白色油狀顆粒。由實驗數據可支持此論點,結塊現象必須在培養基內有正十六烷殘餘時才可能發生並持續進行。 培養過程中培養基酸鹼值的下降,可能使得細胞表面疏水性增加以及細胞間電荷排斥力減少(細胞表面電位上升,在礦物培養基中NTU-1細胞表面帶負電荷)進而幫助結塊現象的發生,但兩者皆不是結塊發生的絕對因子。 本實驗定義之R16值能夠提供簡單判斷結塊現象發生之指標並且利用於石油碳氫化合物污染處裡。 | zh_TW |
dc.description.abstract | Pure culture of Rhodococcus erythropolis NTU-1 was utilized to test the biodegradability and bioflocculate formation in treatment of petroleum pollutant. Emphasis was placed on the factors and mechanism of the formation of cell pellets during the bioremediation process. We found that the main factor of cell pellets formation was the ratio between volume of n-hexadecane and cell density in the growth medium (defined as R16). When the value of R16 was less than 3.5, formation of cell pellets might occur in the culture. Different values of R16 caused differences in morphological structures ranging from yellowish brown particles, flakes, spheres to white oily particles, spheres. The mechanism of cell pellets formation was that n-hexadecane was taken as the linkers between NTU-1 cells and to form the cell pellets by stacking up these cells during the bioremediation process. The bigger of cell pellets, the more n-hexadecane trapped in cell pellets. When the outer layers of cell pellets were covered with NTU-1 cells, the color of cell pellets was yellowish brown. But when the outer layers of cell pellets were covered with n-hexadecane, the color of cell pellets was white and oily. The data support the hypothesis that maximum agglutination of pellets requires the presense of residual amount of n-hexadecane through-out the entire process. During the bioremediation process, pH drop increased of cell surface hydrophobicity and decreased of electrostatic repulsive force among NTU-1 cells (zeta potential of NTU-1 cells of negative charge in the medium increasing from -28mV to -15mV). These changes perhaps helped to form cell pellets, but they are not the only factors of cell pellets formation. The value of R16 provided a simple indicator to morphologies of cell pellets and could be useful in petroleum pollutant treatment. | en |
dc.description.tableofcontents | 摘要 ..................................................................................................................... Ⅰ Abstract ............................................................................................................... Ⅱ 目錄 ..................................................................................................................... Ⅲ 表目錄 ................................................................................................................. Ⅵ 圖目錄 ................................................................................................................. Ⅷ 照片目錄 ............................................................................................................. ⅩⅡ 第一章 導論 .................................................................................. 1 第二章 文獻回顧 .......................................................................... 3 2.1 石油碳氫化合物 .......................................................................................... 3 2.2 石油對環境的影響 ...................................................................................... 4 2.3 石油碳氫化合物移除機制 .......................................................................... 5 2.4 生物降解作用 .............................................................................................. 9 2.4.1 碳氫化合物的利用機制 ........................................................................... 9 2.4.2 碳氫化合物的分解模式 ........................................................................... 14 2.4.3 烷類的降解機制 ....................................................................................... 16 2.4.4 直鏈烷的氧化 ........................................................................................... 18 2.4.5 支鏈烷的氧化 ........................................................................................... 21 2.5菌株Rhodococcus erythropolis介紹 ........................................................... 25 第三章 實驗材料與方法 .............................................................. 30 3.1 實驗菌株 ...................................................................................................... 30 3.2 培養基的組成 .............................................................................................. 32 3.2.1 基礎礦物培養基 ....................................................................................... 32 3.2.2 高緩衝能力礦物培養基 ........................................................................... 34 3.2.3 菌株活化培養基 ....................................................................................... 34 3.2.4 菌株保存培養基 ....................................................................................... 35 3.2.5 計數平板培養基 ....................................................................................... 35 3.2.6 實驗藥品和儀器 ....................................................................................... 36 3.3 實驗方法 ...................................................................................................... 38 3.3.1 菌株的活化和培養 ................................................................................... 38 3.3.2 各種不同實驗條件下正十六烷的測定 ................................................... 40 3.3.3 不同條件下之生物復育實驗 ................................................................... 43 第四章 結果與討論 ...................................................................... 45 4.1含正十六烷礦物培養基中添加Nutrient Broth對NTU-1的生物降解及包覆能力的影響 ........................................................................................... 46 4.1.1 正十六烷礦物培養基初始濃度為1000ppmV ......................................... 46 4.1.2 正十六烷礦物培養基初始濃度為2000ppmV ......................................... 57 4.2 NTU-1在高酸鹼值緩衝能力礦物培養基下添加NB對正十六烷的生物降解及包覆能力的影響 ............................................................................... 66 4.2.1高緩衝能力礦物培養基初始正十六烷濃度為2000ppmV ....................... 66 4.2.2添加NB培養液於高緩衝能力礦物培養基,正十六烷初始濃度為2000ppmV .................................................................................................. 72 4.3 提高培養基NTU-1初始菌量單對於單一菌株NTU-1對正十六烷的生 物降解及包覆能力的影響 ......................................................................... 81 4.4 細胞表面性質的測定 .................................................................................. 88 4.4.1 細胞表面疏水性的測定 ........................................................................... 88 4.4.2 細胞表面電位Zeta potential的測定 ........................................................ 91 4.4.3 在高初始殖菌量和低酸鹼值培養基時,對NTU-1包覆正十六烷能力的影響......................................................................................................... 93 4.5 NTU-1細胞表面狀態對結塊現象的影響 .................................................. 97 4.6 細胞結塊構形和R16值關係之探討 ........................................................... 103 4.7 NTU-1降解直鏈烷之代謝物和碳鏈長之關係 .......................................... 112 第五章 結論 .................................................................................. 117 參考文獻 ........................................................................................... 121 附錄1.................................................................................................. 128 附錄2高初始植菌量條件下,以去離子水取代礦物培養基之正 十六烷生物復育實驗 ........................................................... 131 表目錄 表2.3-1 各種處理程序對於不同污染物的適用性 .......................................... 6 表2.3-2 各種復育技術之特點和應用性 .......................................................... 7 表2.4.1-1 各種有機物在水中之溶解度 ........................................................... 10 表2.4.1-2 菌株E. Lypolttica在降解正烷類的過程中,正烷類溶解度的提升情況 ............................................................................................... 13 表2.4.2-2 以好氧模式分解烷類之微生物 ....................................................... 15 表2.4.2-3 各種生物代謝程序的氧化還原反應式及氧化還原電位 ............... 16 表2.4.3-1 碳氫化合物被微生物降解的難易程度 ........................................... 17 表2.5-2 Rhodococcus菌株聚集性和菌落性質之關係表 ................................ 28 表3.2-1 液態培養基組成表 .............................................................................. 33 表3.2-2 Trace salt solution組成表 ................................................................... 33 表3.2-3 高緩衝能力液態培養基組成表 .......................................................... 34 表3.2-4 菌株保存培養基組成表 ...................................................................... 35 表4.1.1-1 培養條件30℃、100rpm、初始pH值7,培養基中加入不同體積NB培養液,NTU-1處裡1000ppmV時,烷類細胞包覆量(%)和培養基中烷類殘餘量(%) .......................................................... 52 表4.1.2-1 培養條件30℃、100rpm、初始pH值7,培養基中加入不同體積NB培養液,NTU-1處裡2000ppmV時,烷類細胞包覆量(%)和培養基中烷類殘餘量(%) .......................................................... 62 表4.2.1-1 培養條件30℃、100rpm、初始pH值7,不同緩衝能力培養基,NTU-1處裡2000ppmV時,烷類細胞包覆量(%)和培養基中烷類殘餘量(%) .................................................................................... 70 表4.2.2-1 培養條件30℃、100rpm、初始pH值7,高緩衝能力培養基中加入不同體積NB培養液,NTU-1處裡2000ppmV時,烷類細胞包覆量(%)和培養基中烷類殘餘量(%) ...................................... 77 表4.3-1 培養條件30℃、100rpm、初始pH值7,高初始植菌量培養基,NTU-1處裡1000ppmV時,烷類細胞包覆量(%)和培養基中烷類殘餘量(%) ........................................................................................... 86 表4.4.2-1 計算表面電位之Smoluchowski equation ....................................... 91 表4.5-1 培養條件30℃、100rpm、初始pH值7,不同細胞表面狀態,NTU-1處裡正十六烷,烷類細胞包覆量(%)和培養基中烷類殘餘量(%) ....................................................................................................... 100 表4.6-1 培養過程中,結塊現象發生時之R16值和結塊構型圖表 ............... 104 表4.6-2 在顆粒狀結塊沉澱形成時,添加正十六烷於培養基中,結塊構形 的變化情形 ......................................................................................... 1105 表4.7-1 NTU-1降解不同直鏈烷時,培養基內氫離子釋放量和生物降解量之關係式 ............................................................................................... 114 附錄表-1 培養條件30℃、100rpm、初始pH值7.3,以去離子水取代礦物培養基,NTU-1處裡正十六烷,培養十天後之生物降解量、包覆量及總移除量表格 ....................................................................... 133 圖目錄 圖2.4.1-1 微胞粒子的結構示意圖 ................................................................... 12 圖2.4.2-1 碳氫化合物分解流程圖,實線代表carbon flux、虛線代表O2 flux及電子接收者 ................................................................................... 14 圖2.4.4-1 正直鏈烷(n-alkane)的降解及代謝路徑 ......................................... 19 圖2.4.4-2 雙末端氧化屬於單末端氧化的副代謝路徑 ................................... 20 圖2.4.4-3 次末端氧化代謝路徑 ....................................................................... 21 圖2.4.5-1菌株Corynebacterium sp.對異十九烷之代謝途徑 .......................... 22 圖2.4.5-2 菌株Brevibacterium erythrogenes對於異十九烷的代謝途徑 ...... 23 圖2.4.5-3 無孢子放射菌對於各種碳氫化合物之代謝途徑 ........................... 24 圖2.5-1 由Rhodococcus催化發生的各種不同的氧化反應 ........................... 26 圖3.3.1-1 計數平板培養基之使用及計算 ....................................................... 40 圖4.1.1-1 培養條件30℃、100rpm、初始pH值7,培養基中加入不同體積NB培養液,NTU-1處裡1000ppmV正十六烷之酸鹼值變化 ..................................................................................................... 47 圖4.1.1-2 E. coli利用glucose和lactose生長實驗中,細胞生長和碳源濃度的關係變化 ....................................................................................... 48 圖4.1.1-3 培養條件30℃、100rpm、初始pH值7,培養基中加入不同體積NB培養液,NTU-1處裡1000ppmV正十六烷之細胞生長趨勢 ..................................................................................................... 49 圖4.1.1-4 烷類被細胞包覆量、生物降解量、總移除量、殘餘在培養基中之量的計算方法及定義之示意圖 ................................................... 51 圖4.1.1-5培養條件30℃、100rpm、初始pH值7,培養基中加入不同體積NB培養液,NTU-1處裡1000ppmV正十六烷之生物降解量 ..................................................................................................... 52 圖4.1.2-1 培養條件30℃、100rpm、初始pH值7,培養基中加入不同體積NB培養液,NTU-1處裡2000ppmV正十六烷時之酸鹼值變化 ..................................................................................................... 58 圖4.1.2-2 培養條件30℃、100rpm、初始pH值7,培養基中加入不同體積NB培養液,NTU-1處裡2000ppmV正十六烷時之細胞生長趨勢 ................................................................................................. 60 圖4.1.2-3 培養條件30℃、100rpm、初始pH值7,培養基中加入不同體積NB培養液,NTU-1處裡1000ppmV正十六烷時之生物降解量 ..................................................................................................... 61 圖4.2.1-1 培養條件30℃、100rpm、初始pH值7,不同緩衝能力培養基, NTU-1處裡2000ppmV正十六烷時之酸鹼值變化 ....................... 67 圖4.2.1-2 培養條件30℃、100rpm、初始pH值7,不同緩衝能力培養基,NTU-1處裡2000ppmV正十六烷時之生長趨勢 ......................... 68 圖4.2.1-3 培養條件30℃、100rpm、初始pH值7,不同緩衝能力培養基,NTU-1處裡2000ppmV正十六烷時之生物降解量 ..................... 69 圖4.2.2-1培養條件30℃、100rpm、初始pH值7,高緩衝能力培養基中加入不同體積NB培養液,NTU-1處裡2000ppmV正十六烷時之酸鹼值變化 ................................................................................. 73 圖4.2.2-2 培養條件30℃、100rpm、初始pH值7,高緩衝能力培養基中加入不同體積NB培養液,NTU-1處裡2000ppmV正十六烷時之生長趨勢 ..................................................................................... 75 圖4.2.2-3 培養條件30℃、100rpm、初始pH值7,高緩衝能力培養基中加入不同體積NB培養液,NTU-1處裡2000ppmV正十六烷時之生物降解量 ................................................................................. 76 圖4.3-1 培養條件30℃、100rpm、初始pH值7,高初始植菌量培養基,NTU-1處裡1000ppmV正十六烷之酸鹼值變化 ........................... 82 圖4.3-2 培養條件30℃、100rpm、初始pH值7,高初始植菌量培養基,NTU-1處裡1000ppmV正十六烷之細胞生長趨勢圖 ................... 83 圖4.3-3 培養條件30℃、100rpm、初始pH值7,高初始植菌量培養基,NTU-1處裡1000ppmV正十六烷之生物降解量圖 ....................... 84 圖4.4.1-1 MATH實驗之簡單實驗流程 ........................................................... 89 圖4.4.1-2 MATH在不同條件下之實驗結果 ................................................... 89 圖4.4.2-1 培養基在不同酸鹼值下,NTU-1細胞表面電位(zeta-potential)的變化 ............................................................................................... 92 圖4.4.3-1 培養條件30℃、100rpm、初始pH值4.5,高初始植菌量培養基,NTU-1處裡正十六烷之生物降解量 .......................................... 94 圖4.5-1 培養條件30℃、100rpm、初始pH值7,不同細胞表面狀態,NTU-1處裡正十六烷之生長趨勢圖 ............................................................. 98 圖4.5-2 培養條件30℃、100rpm、初始pH值7,不同細胞表面狀態,NTU-1處裡正十六烷之生物降解量 ............................................................. 99 圖4.6-1 NTU-1細胞以正十六烷為linker形成結塊之機制示意圖 ............... 108 圖4.6-2 R16值=0.8-1.2時,結塊機制示意圖(培養基內還有未貼附於正十六烷之細胞) ............................................................................................ 109 圖4.6-3 R16值=1.9時,結塊機制示意圖(培養基內無未貼附於正十六烷之細胞) .................................................................................................... 109 圖4.6-4 R16值=2.7-3.2時,結塊機制示意圖(以正十六烷作為linker) ............ 110 圖4.7-1 培養條件30℃、100rpm、初始pH值7,NTU-1降解正十六烷,釋放於培養基氫離子量與生物降解量關係圖 ................................. 112 圖4.7-2 培養條件30℃、100rpm、初始pH 值7,NTU-1降解正十八烷,釋放於培養基氫離子量與生物降解量關係圖 ................................. 113 圖4.7-3 培養條件30℃、100rpm、初始pH值7,在高和低緩衝能力培養基中,NTU-1降解正十六烷,釋放於培養基氫離子量與生物降解量關係圖 ............................................................................................. 115 圖4.7-4 不同緩衝能力培養基內NTU-1降解直鏈烷類時,氫離子釋放量與生物降解量關係圖 ......................................................................... 116 附圖(A) NTU-1 菌株在Nutrient Broth中的生長曲線圖 ........................... 128 附圖(B) 波長600nm下的OD值與細胞乾重關係圖 ...................................... 128 附圖(C) 正十六烷在氣相層析儀中之校正曲線 ............................................. 129 附圖(D) 低緩衝能力礦物培養基之酸鹼值滴定曲線圖 ................................. 129 附圖(E) 高酸鹼緩衝能力礦物培養基之酸鹼值滴定曲線圖 ......................... 130 照片目錄 照片3.1-1 顯微鏡下的Rhodococcus erythropolis NTU-1 ............................... 31 照片3.1-2 計數平板培養基內Rhodococcus erythropolis NTU-1菌落 ......... 31 照片4.1.1-1 培養條件30℃、100rpm、初始pH值7,培養基中未加入NB培養液,NTU-1處裡1000ppmV正十六烷,在44小時所出現的淡黃色片狀結塊現象 ......................................................... 54 照片4.1.1-2(a) 培養條件30℃、100rpm、初始pH值7,培養基中加入3 NB培養液,NTU-1處裡1000ppmV正十六烷,培養至32小時出現之鬆散土黃色顆粒結塊 ............................................. 55 照片4.1.1-2(b) 培養條件30℃、100rpm、初始pH值7,培養基中加入3 NB培養液,NTU-1處裡1000ppmV正十六烷,培養至44小時出現之淡黃色片狀結塊 ..................................................... 55 照片4.1.1-2(c) 培養條件30℃、100rpm、初始pH值7,培養基中加入3 NB培養液,NTU-1處裡1000ppmV正十六烷,培養至44小時出現之立體結塊顆粒結塊 ................................................. 55 照片4.1.1-3(a) 培養條件30℃、100rpm、初始pH值7,培養基中加入5ml NB培養液,NTU-1處裡1000ppmV正十六烷,培養至32小時出現之鬆散土黃色顆粒結塊 ......................................... 56 照片4.1.1-3(b) 培養條件30℃、100rpm、初始pH值7,培養基中加入5ml NB培養液,NTU-1處裡1000ppmV正十六烷,培養至44小時出現之立體結塊顆粒結塊 ............................................. 56 照片4.1.2-1 培養條件30℃、100rpm、初始pH值7,培養基中未加入 NB培養液,NTU-1處裡2000ppmV正十六烷,在56小時所出現的乳白色顆粒結塊 ................................................................. 63 照片4.1.2-2 培養條件30℃、100rpm、初始pH值7,培養基中加入1ml NB培養液,NTU-1處裡2000ppmV正十六烷,在56小時所出現的土黃色片狀結塊 ................................................................. 64 照片4.1.2-3 培養條件30℃、100rpm、初始pH值7,培養基中加入2ml NB培養液,NTU-1處裡2000ppmV正十六烷,在32小時出現的結塊現象 ................................................................................. 64 照片4.1.2-4 培養條件30℃、100rpm、初始pH 值7,培養基中加入3ml NB培養液,NTU-1處裡2000ppmV正十六烷,在32小時所出現的乳白色圓球狀結塊 ..................................................... 65 照片4.2.1-1 培養條件30℃、100rpm、初始pH值7,高緩衝能力培養基,NTU-1處裡2000ppmV正十六烷,在56小時所出現的白色絲狀聚集現象 ............................................................................. 71 照片4.2.1-2 培養條件30℃、100rpm、初始pH值7,高緩衝能力培養基, NTU-1處裡2000ppmV正十六烷,在68小時所出現的白色顆粒狀結塊現象 ......................................................................... 72 照片4.2.2-1(a) 培養條件30℃、100rpm、初始pH值7,培養基內加入1ml NB培養液,NTU-1處裡2000ppmV正十六烷,培養至56小時所出現之土黃色顆粒狀結塊 ......................................... 78 照片4.2.2-1(b) 培養條件30℃、100rpm、初始pH值7,培養基內加入1ml NB培養液,NTU-1處裡2000ppmV正十六烷,培養至92小時,部分土黃色顆粒沉澱之情形 ....................................... 78 照片4.2.2-2(a) 培養條件30℃、100rpm、初始pH值7,培養基內加入3ml NB培養液,NTU-1處裡2000ppmV正十六烷,培養至56小時所出現之土黃色顆粒狀結塊 ......................................... 79 照片4.2.2-2(b) 培養條件30℃、100rpm、初始pH值7,培養基內加入3ml NB培養液,NTU-1處裡2000ppmV正十六烷,培養至92小時,土黃色顆粒皆沉澱至培養基底部 ............................... 79 照片4.2.2-3(a) 培養條件30℃、100rpm、初始pH值7,培養基中添加5ml NB培養液,NTU-1處裡2000ppmV正十六烷,培養至44小時出現之土黃色顆粒(粉狀)狀結塊 .................................. 80 照片4.2.2-3(b) 培養條件30℃、100rpm、初始pH值7,培養基中添加5ml NB培養液,NTU-1處裡2000ppmV正十六烷,培養至56小時後,土黃色顆粒皆沉澱至培養基底部............................. 80 照片4.3-1(a) 培養條件30℃、100rpm、初始pH值7,高初始植菌量培養基(A)組,NTU-1處裡1000ppmV正十六烷,培養至18小時出現的土黃色顆粒結塊 ............................................................. 86 照片4.3-1(b) 培養條件30℃、100rpm、初始pH值7,高初始植菌量培養基(A)組,NTU-1處裡1000ppmV正十六烷,培養至32小時出現的結塊沉澱 ......................................................................... 86 照片4.3-2(a) 培養條件30℃、100rpm、初始pH值7,高初始植菌量培養基(B)組,NTU-1處裡1000ppmV正十六烷,培養至28小時出現的土黃色顆粒結塊 ............................................................. 87 照片4.3-2(b) 培養條件30℃、100rpm、初始pH值7,高初始植菌量培養基(B)組,NTU-1處裡1000ppmV正十六烷,在44小時出現的結塊沉澱 ................................................................................. 87 照片4.4.1-1 MATH在靜置3小時後,NTU-1貼附於正十六烷之情況 .......... 90 照片4.4.3-1 培養條件30℃、100rpm、初始pH值4.5,培養基(R16)Int = 2.208,培養基未添加NB培養液,NTU-1處裡正十六烷,培養20小時出現之聚集現象 .......................................................... 96 照片4.4.3-2 培養條件30℃、100rpm、初始pH值4.5,培養基(R16)Int = 2.208,培養基未添加NB培養液,NTU-1處裡正十六烷,培養56小時貼附於瓶壁的聚集現象 .............................................. 96 照片4.5-1 培養條件30℃、100rpm、初始pH值7,不同細胞表面狀態,培養基未添加NB培養液,NTU-1處裡正十六烷,培養至56小時出現之白色球狀結塊 ........................................................... 101 照片4.5-2(a) 培養條件30℃、100rpm、初始pH值7,不同細胞表面狀態,培養基未添加NB培養液,NTU-1處裡正十六烷,培養至17小時即出現之白色球狀結塊 ................................................ 102 照片4.5-2(b) 培養條件30℃、100rpm、初始pH值7,不同細胞表面狀態,培養基未添加NB培養液,NTU-1處裡正十六烷,培養至32、68小時出現之淡黃色球狀結塊 .......................................... 102 照片4.5-2(c) 培養條件30℃、100rpm、初始pH值7,不同細胞表面狀態,培養基未添加NB培養液,NTU-1處裡正十六烷,培養至44小時出現之黃色顆粒狀結塊 ................................................ 102 照片4.5-2(d) 培養條件30℃、100rpm、初始pH值7,不同細胞表面狀態,培養基未添加NB培養液,NTU-1處裡正十六烷,培養至56小時出現之淡黃色顆粒沉澱 .............................................. 102 照片4.6-1 培養基R16值大於4時,出現之白色絲狀聚集物 .......................... 111 附錄照片-1(a) 培養條件30℃、100rpm、初始pH值7.3,以去離子水取代礦物培養基,初始植菌量為0.113g,NTU-1處裡正十六烷,培養至3天出現之聚集現象 .............................................. 132 附錄照片-1(a) 培養條件30℃、100rpm、初始pH值7.3,以去離子水取代礦物培養基,初始植菌量為0.113g,NTU-1處裡正十六烷,培養至10天出現之土黃色顆粒結塊現象 ........................ 132 附錄照片-2(a) 培養條件30℃、100rpm、初始pH值7.3,以去離子水取代礦物培養基,初始植菌量為0.1291g,NTU-1處裡正十六烷,培養至3天出現之聚集現象 .............................................. 133 附錄照片-2(a) 培養條件30℃、100rpm、初始pH值7.3,以去離子水取代礦物培養基,初始植菌量為0.1291g,NTU-1處裡正十六烷,培養至10天出現之土黃色顆粒結塊現象 ........................ 133 | zh_TW |
dc.format.extent | 6578873 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | en |
dc.language.iso | en_US | - |
dc.subject | 生物復育 | en |
dc.subject | 正十六烷 | en |
dc.subject | 疏水性 | en |
dc.subject | Rhodococcus erythropolis | en |
dc.subject | bioremediation | en |
dc.subject | petroleum treatment | en |
dc.subject | hydrophobicity | en |
dc.subject.classification | [SDGs]SDG11 | - |
dc.title | 微生物生物復育過程中細胞聚集現象之研究 | zh |
dc.title | Cell Pellets Formation in Microbial Bioremediation | en |
dc.type | thesis | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/52281/1/ntu-96-R94524064-1.pdf | - |
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