|標題:||利用混合菌株（TN-4）與單一菌株（Rhodococcus erythropolis NTU-1）處理正十六烷之研究
Bioremediation of n-hexadecane by mixed culture TN-4 and pure culture Rhodococcus erythropolis NTU-1
|關鍵字:||生物降解;正十六;烷細菌結塊;bioremediation;n-hexadecane;bioflocculate||公開日期:||2005||摘要:||石油為世界上主要的能源, 但同時也給人類生存的環境帶來了嚴重污染，因此要如何降低石油碳氫化合物對環境的影響是非常重要的。本研究主要探討TN-4混合菌株與Rhodococcus erythropolis NTU-1單一菌株在（1）不同正十六烷濃度與（2）不同初始培養基酸鹼值下對正十六烷所呈現的生物降解能力以及烷類包覆能力。
Petroleum is one of the world’s major resources of energy. Nevertheless, continual exploitation of petroleum hydrocarbon has severely polluted our environment and threatened the well being of human life. The major purpose of this research was to investigate n-hexadecane biodegradability and bioflocculate formation performed by a mixed culture TN-4 and a pure culture Rhodococcus erythropolis NTU-1. Two key abiotic factors (1) n-hexadecane concentration and (2) initial acidity of culture medium, were altered to carry out the research work. It was discovered that the growth of TN-4 increased as the concentration of n-hexadecane rises even though the alkane consumption ability performed was fairly alike especially under high substrate concentration. However, both growth and biodegradability of NTU-1 was not affected for culture under different concentration of n-hexadecane. In addition, TN-4 and NTU-1 attained the best alkane removal efficiency when the initial acidity of culture medium was adjusted to pH 7.5. Both strains successfully removed almost 100% of n-hexadecane (up to 1470 ppm) after incubation for 66 hours. The bioremediation process was accompanied by formation of bacterial pellets (bioflocculate), with size ranging from 0.1 to 2 cm in diameter. This flocculation mechanism had provided high removal efficiency as most of the residual n-hexadecane was engulfed by the bacterial pellets and being consequently biodegraded. Aggregates formed by TN-4 are yellowish, round in shape and relatively firmer. Aggregates formed by NTU-1 are white in color and much incompact in comparison. Furthermore, it was also discovered that further addition of hexanoic acid into the culture could have possibly modified the surface properties of the cell because the flocs formed during incubation tended to attach to each other furing incubation. Besides that, due to the comparable results shown by these two strains, it was postulated that NTU-1 played a primary role of n-hexadecane degradation as well as bioflocculate formation throughout the process. Last but not least, development of bioflocculate was non trivial due to its importance in the enhancement of alkane mediation as well as easy removal of suspended solids in wastewater treatment.
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