https://scholars.lib.ntu.edu.tw/handle/123456789/626401
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Li, Ching-Hao | en_US |
dc.contributor.author | MING-KWANG SHYU | en_US |
dc.contributor.author | Jhan, Cheng | en_US |
dc.contributor.author | Cheng, Yu-Wen | en_US |
dc.contributor.author | Tsai, Chi-Hao | en_US |
dc.contributor.author | Liu, Chen-Wei | en_US |
dc.contributor.author | Lee, Chen-Chen | en_US |
dc.contributor.author | Chen, Ruei-Ming | en_US |
dc.contributor.author | JAW-JOU KANG | en_US |
dc.date.accessioned | 2022-12-14T05:44:58Z | - |
dc.date.available | 2022-12-14T05:44:58Z | - |
dc.date.issued | 2015-11 | - |
dc.identifier.issn | 1096-6080 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/626401 | - |
dc.description.abstract | Gold nanoparticles (Au-NPs) are being increasingly used as constituents in cosmetics, biosensors, bioimaging, photothermal therapy, and targeted drug delivery. This elevated exposure to Au-NPs poses systemic risks in humans, particularly risks associated with the biodistribution of Au-NPs and their potent interaction with biological barriers. We treated human umbilical vein endothelial cells with Au-NPs and comprehensively examined the expression levels of tight junction (TJ) proteins such as occludin, claudin-5, junctional adhesion molecules, and zonula occludens-1 (ZO-1), as well as endothelial paracellular permeability and the intracellular signaling required for TJ organization. Moreover, we validated the effects of Au-NPs on the integrity of TJs in mouse brain microvascular endothelial cells in vitro and obtained direct evidence of their influence on blood-brain barrier (BBB) permeability in vivo. Treatment with Au-NPs caused a pronounced reduction of PKCζ-dependent threonine phosphorylation of occludin and ZO-1, which resulted in the instability of endothelial TJs and led to proteasome-mediated degradation of TJ components. This impairment in the assembly of TJs between endothelial cells increased the permeability of the transendothelial paracellular passage and the BBB. Au-NPs increased endothelial paracellular permeability in vitro and elevated BBB permeability in vivo. Future studies must investigate the direct and indirect toxicity caused by Au-NP-induced endothelial TJ opening and thereby address the double-edged-sword effect of Au-NPs. | en_US |
dc.language.iso | en | en_US |
dc.publisher | OXFORD UNIV PRESS | en_US |
dc.relation.ispartof | Toxicological sciences : an official journal of the Society of Toxicology | en_US |
dc.subject | blood-brain barrier; endothelial barrier; paracellular permeability; protein kinase C zeta (PKCζ); tight junction | en_US |
dc.title | Gold Nanoparticles Increase Endothelial Paracellular Permeability by Altering Components of Endothelial Tight Junctions, and Increase Blood-Brain Barrier Permeability in Mice | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1093/toxsci/kfv176 | - |
dc.identifier.pmid | 26272951 | - |
dc.identifier.scopus | 2-s2.0-84947779009 | - |
dc.identifier.isi | WOS:000365547900017 | - |
dc.identifier.url | https://scholars.lib.ntu.edu.tw/handle/123456789/392608 | - |
dc.relation.pages | 192 | en_US |
dc.relation.journalvolume | 148 | en_US |
dc.relation.journalissue | 1 | en_US |
dc.relation.pageend | 203 | en_US |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | National Taiwan University Hospital Yun-Lin Branch | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHYL | - |
crisitem.author.orcid | 0000-0001-9057-4101 | - |
crisitem.author.orcid | 0000-0002-6350-3997 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital Yun-Lin Branch | - |
顯示於: | 醫學系 |
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