https://scholars.lib.ntu.edu.tw/handle/123456789/627168
標題: | Sequential Approach to Improve the Molecular Classification of Childhood Acute Lymphoblastic Leukemia | 作者: | Yu, Chih-Hsiang Wu, Gang Chang, Chia-Ching SHIANN-TANG JOU MENG-YAO LU Lin, Kai-Hsin Chen, Shu-Huey Wu, Kang-Hsi Huang, Fang-Liang Cheng, Chao-Neng HSIU-HAO CHANG Hedges, Dale Wang, Jinn-Li Yen, Hsiu-Ju MENG-JU LI SHU-WEI CHOU Hung, Chen-Ting Lin, Ze-Shiang Lin, Chien-Yu Chen, Hsuan-Yu Ni, Yu-Ling Hsu, Yin-Chen Lin, Dong-Tsamn SHU-WHA LIN Yang, Jun J Pui, Ching-Hon SUNG-LIANG YU YUNG-LI YANG |
公開日期: | 十一月-2022 | 卷: | 24 | 期: | 11 | 來源出版物: | The Journal of molecular diagnostics : JMD | 摘要: | Identification of specific leukemia subtypes is a key to successful risk-directed therapy in childhood acute lymphoblastic leukemia (ALL). Although RNA sequencing (RNA-seq) is the best approach to identify virtually all specific leukemia subtypes, the routine use of this method is too costly for patients in resource-limited countries. This study enrolled 295 patients with pediatric ALL from 2010 to 2020. Routine screening could identify major cytogenetic alterations in approximately 69% of B-cell ALL (B-ALL) cases by RT-PCR, DNA index, and multiplex ligation-dependent probe amplification. STIL-TAL1 was present in 33% of T-cell ALL (T-ALL) cases. The remaining samples were submitted for RNA-seq. More than 96% of B-ALL cases and 74% of T-ALL cases could be identified based on the current molecular classification using this sequential approach. Patients with Philadelphia chromosome-like ALL constituted only 2.4% of the entire cohort, a rate even lower than those with ZNF384-rearranged (4.8%), DUX4-rearranged (6%), and Philadelphia chromosome-positive (4.4%) ALL. Patients with ETV6-RUNX1, high hyperdiploidy, PAX5 alteration, and DUX4 rearrangement had favorable prognosis, whereas those with hypodiploid and KMT2A and MEF2D rearrangement ALL had unfavorable outcomes. With the use of multiplex ligation-dependent probe amplification, DNA index, and RT-PCR in B-ALL and RT-PCR in T-ALL followed by RNA-seq, childhood ALL can be better classified to improve clinical assessments. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/627168 | ISSN: | 15251578 | DOI: | 10.1016/j.jmoldx.2022.08.001 |
顯示於: | 醫學系 |
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