https://scholars.lib.ntu.edu.tw/handle/123456789/627779
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Kondo, Yasuteru | en_US |
dc.contributor.author | Sung, Vicky M H | en_US |
dc.contributor.author | Machida, Keigo | en_US |
dc.contributor.author | HELENE MINYI LIU | en_US |
dc.contributor.author | Lai, Michael M C | en_US |
dc.date.accessioned | 2023-02-08T07:22:58Z | - |
dc.date.available | 2023-02-08T07:22:58Z | - |
dc.date.issued | 2007-04-25 | - |
dc.identifier.issn | 0042-6822 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/627779 | - |
dc.description.abstract | It has been reported that hepatitis C virus (HCV) may infect and replicate in human T cells, particularly in perihepatic lymph nodes, but the extent and consequence of T-cell infection in patients is unclear. This study is conducted to characterize the parameters and functional consequences of HCV infection in T lymphocytes. By using a lymphotropic HCV strain, we showed that HCV could infect T cell lines (Molt-4 and Jurkat cells) in vitro. Both positive- and negative-strand HCV RNA were detected for several weeks after infection. Viral proteins could also be detected by immunofluorescence studies. Moreover, infectious HCV particles were produced from Molt-4 cell cultures, and could be used to infect naïve T cell lines. HCV could also infect human primary CD4+ T cells, particularly naïve (CD45RA+CD45RO-) CD4+ cells, in culture. The amounts of STAT-1 and phosphorylated STAT-1 proteins in the infected Molt-4 cells were significantly less than those in uninfected cultures, suggesting the possibility of defect in interferon-gamma signaling. Indeed, T-bet and STAT-1 mRNA levels after interferon-gamma stimulation in infected Molt-4 were suppressed. In conclusion, HCV could infect and transiently replicate in T cells and that HCV replication suppressed the IFN-gamma/STAT-1/T-bet signaling due to the reduction of STAT-1 and inhibition of its activation (phosphorylation). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | en_US |
dc.relation.ispartof | Virology | en_US |
dc.subject | CD4; CD45RA; STAT-1; T-bet; lymphotropism; HCV; POLYMERASE-CHAIN-REACTION; BLOOD MONONUCLEAR-CELLS; IN-VITRO; LIVER INFLAMMATION; IMMUNE-RESPONSES; RNA REPLICATION; HCV; EXPRESSION; CD81; LYMPHOCYTES | en_US |
dc.title | Hepatitis C virus infects T cells and affects interferon-gamma signaling in T cell lines | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.virol.2006.11.009 | - |
dc.identifier.pmid | 17175001 | - |
dc.identifier.scopus | 2-s2.0-34047261821 | - |
dc.identifier.isi | WOS:000246042300017 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/34047261821 | - |
dc.relation.pages | 161 | en_US |
dc.relation.journalvolume | 361 | en_US |
dc.relation.journalissue | 1 | en_US |
dc.relation.pageend | 173 | en_US |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Biochemistry and Molecular Biology | - |
crisitem.author.orcid | 0000-0003-4837-5373 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 生物化學暨分子生物學科研究所 |
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