Rapid and sensitive detection of CALR exon 9 mutations using high-resolution melting analysis
Journal
Clinica chimica acta; international journal of clinical chemistry
Journal Volume
440
Pages
133
Date Issued
2015-02-02
Author(s)
Lim, Ken-Hong
Lin, Huan-Chau
Chen, Caleb Gon-Shen
Wang, Wei-Ting
Chang, Yu-Cheng
Chiang, Yi-Hao
Lin, Ching-Sung
Su, Nai-Wen
Su, Ying-Wen
Lin, Johnson
Chang, Yi-Fang
Chang, Ming-Chih
Hsieh, Ruey-Kuen
Abstract
Somatic CALR exon 9 mutations have recently been identified in patients with JAK2/MPL-unmutated myeloproliferative neoplasm, and have become an important clonal marker for the diagnosis of essential thrombocythemia (ET) and primary myelofibrosis. In the present study, we sought to use high-resolution melting analysis (HRMA) as a screening method for the detection of CALR mutations.
Subjects
Calreticulin (CALR); Essential thrombocythemia; High-resolution melting analysis; Mutation; Myeloproliferative neoplasm
Publisher
ELSEVIER SCIENCE BV
Type
journal article