https://scholars.lib.ntu.edu.tw/handle/123456789/62844
標題: | Polyglycolic acid/chitosan glue and apoptosis of endometriotic cells | 作者: | Wang, Yu-Chi Fu, Ru-Huei Hsieh, Hsyue-Jen Chao, Hsiang-Tai Kao, Shu-Huei |
關鍵字: | Apoptosis; Chitosan; Endometriosis; Polyglycolic acid | 公開日期: | 2005 | 卷: | 84 | 期: | 1 | 起(迄)頁: | 75-81 | 來源出版物: | Fertility and Sterility | 摘要: | Objective: To induce apoptosis of endometriotic cells of patients with endometriosis. Design: To demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis. Setting: University hospital infertility center. Patient(s): Twelve women who visited the center for infertility therapy. Intervention(s): Polyglycolic acid/chitosan glue was applied into primary endometriotic cells; the manipulated cells were collected 1-4 days after polyglycolic acid/chitosan glue treatment. Main Outcome Measure(s): Primary endometriotic cell cultures from eutopic endometriotic tissue were established. The effect of the novel biological glue, polyglycolic acid/chitosan glue A, on endometrial cells in vitro was examined. The different stages of apoptosis were analyzed using flow cytometry with fluorescein isothiocyanate conjugate (FITC)-annexin V and propidium iodide staining. Result(s): The growth inhibitory effects of polyglycolic acid/chitosan glue A on endometrial cells were found to be dose-response and time dependent. Less than 15% viability was detected in cultures containing 2,000 μg of polyglycolic acid/chitosan glue A after 4 days of treatment. Induced apoptosis and caspase activity were revealed. The caspase-3 activity increased 2.2-fold with 4 days of culture with 2,000 μg of polyglycolic acid/chitosan glue A. Conclusion(s): This is the first study to demonstrate that polyglycolic acid/chitosan glue directly inhibits cell proliferation by inducing apoptosis, thus suggesting that this new biological glue may be useful for endometriosis therapy. ?2005 by American Society for Reproductive Medicine. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/87248 | DOI: | 10.1016/j.fertnstert.2005.01.127 | SDG/關鍵字: | caspase 3; chitosan; fluorescein isothiocyanate; glue; lipocortin 5; polyglycolic acid; propidium iodide; apoptosis; article; cell culture; cell proliferation; cell viability; clinical examination; conjugate; controlled study; dose response; endometriosis; endometrium cell; enzyme activity; female; flow cytometry; human; human cell; human tissue; infertility therapy; priority journal; staining; time; university hospital; Apoptosis; Cell Proliferation; Cells, Cultured; Chitosan; Dose-Response Relationship, Drug; Endometriosis; Endometrium; Female; Humans; Polyglycolic Acid |
顯示於: | 化學工程學系 |
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