https://scholars.lib.ntu.edu.tw/handle/123456789/628812
標題: | Expression of SnoRNA U50A Is Associated with Better Prognosis and Prolonged Mitosis in Breast Cancer | 作者: | Li, Jie-Ning MING-YANG WANG Chen, Yi-Ting Kuo, Yao-Lung Chen, Pai-Sheng |
關鍵字: | breast cancer; cancer prognosis; mitosis; snoRNA | 公開日期: | 15-十二月-2021 | 出版社: | MDPI | 卷: | 13 | 期: | 24 | 來源出版物: | Cancers | 摘要: | Small nucleolar RNAs (snoRNAs) are small noncoding RNAs generally recognized as housekeeping genes. Genomic analysis has shown that snoRNA U50A (U50A) is a candidate tumor suppressor gene deleted in less than 10% of breast cancer patients. To date, the pathological roles of U50A in cancer, including its clinical significance and its regulatory impact at the molecular level, are not well-defined. Here, we quantified the copy number of U50A in human breast cancer tissues. Our results showed that the U50A expression level is correlated with better prognosis in breast cancer patients. Utilizing RNA-sequencing for transcriptomic analysis, we revealed that U50A downregulates mitosis-related genes leading to arrested cancer cell mitosis and suppressed colony-forming ability. Moreover, in support of the impacts of U50A in prolonging mitosis and inhibiting clonogenic activity, breast cancer tissues with higher U50A expression exhibit accumulated mitotic tumor cells. In conclusion, based on the evidence from U50A-downregulated mitosis-related genes, prolonged mitosis, repressed colony-forming ability, and clinical analyses, we demonstrated molecular insights into the pathological impact of snoRNA U50A in human breast cancer. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/628812 | ISSN: | 2072-6694 | DOI: | 10.3390/cancers13246304 |
顯示於: | 醫學院附設癌醫中心醫院(臺大癌醫) |
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